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Item Identifying promoters to enhance heterologous gene expression in recombinant saccharomyces cerevisiae strains cultivated on non-native substrates(Springer Science and Business Media Deutschland GmbH, 2025) Fortuin, Jordan; den Haan, RiaanAbstract: Efficient bioconversion of lignocellulosic biomass (LCB) to ethanol by Saccharomyces cerevisiae requires its engineering to express heterologous enzymes at titres high enough to make significant impacts on industrial consolidated bioprocessing (CBP). Promoters are required for this purpose, but are reportedly influenced by various environmental factors as well as the protein specific nature of expression, warranting the need for assessment under the conditions for which they are intended. Heterologous xylosidase- and xylanase-encoding genes (xln43_SED1 and xyn2) were individually cloned under transcriptional control of the SED1P and TDH3P promoters, and DIT1T terminator, and integrated into the genome of an a S. cerevisiae strain engineered for xylose utilization. Enzymatic assays were used to quantify the performance of the promoters when strains were cultivated on glucose (aerobically and micro-aerobically) and xylose. Additional strains containing both xln43_SED1 and xyn2 under different promoter combinations were then used to allow direct fermentation of beechwood xylan to ethanol in a CBP. The SED1P/DIT1T and TDH3P/DIT1T combinations significantly outperformed the benchmark ENO1P/T under all of the tested cultivation conditions, as well as with regard to growth trials on non-native substrates (xylo-oligosaccharides/XOS and beechwood xylan) and fermentations of beechwood xylan to ethanol. Overall, TDH3P was the best-performing promoter. This study demonstrates that heterologous metabolic pathways and CBP can be significantly enhanced by employing carefully selected promoters tailored to specific conditions.Item Characterization of Zalaria obscura Y1223 hydrolases: implications for lignocellulose conversion(Springer Science and Business Media Deutschland GmbH, 2025) Boonzaier, Jeremy J.; den Haan, Riaan; Hart, Rodney S.Overconsumption of fossil fuel reserves and its adverse effects has sparked interest in the production of second-generation biofuels due to the abundance of lignocellulosic waste and potential energy crops. However, processing costs associated with depolymerization of the cellulose crystalline structure have stalled advancement in cellulosic ethanol production. Current investigations range from identification of novel enzymes for lignocellulose hydrolysis to consolidation of enzyme production into a singular alcohol producing microorganism to potentially reduce cost for commercial processing. In this study, a total of 828 non-Saccharomyces and black yeasts were screened for cellulolytic and xylanolytic enzyme activities, whereby 60 isolates were identified that exhibited activity for at least one of the enzymes tested. In doing so, a novel Zalaria obscura strain (Z. obscura Y1223) was identified and assessed for enzyme activity in multiple growth media. Semi-quantitative assays showed that Z. obscura Y1223 produced cellulases optimally in media containing yeast extract, peptone and oat bran, with a pH range between pH 5 and 6 and at 30 °C. Maximum xylanase activity (20.5 U/L/OD600) was attained using synthetic complete media supplemented with xylo-oligosaccharides and maximum cellulase activity (7.51 U/L/OD600 endoglucanase, 1.302 U/L/OD600 β-glucosidase) was attained when grown in media containing yeast extract, peptone and oat bran. To our knowledge, this is the first study to quantify the cellulolytic and xylanolytic enzyme activities of a Zalaria spp., which provides key insight into the availability of unexplored cellulolytic enzymes that could inform the design of organisms engineered for consolidated bioprocessing.Item Ferrocene-based hybrid drugs as potential anticancer and antibacterial therapeutic agents for incorporation into nanocarriers: in silico, in vitro, molecular docking evaluations(MDPI AG, 2025) Oselusi Samson; Peter Sijongesonke; Morifi Eric; Nwamadi MutshinyaloCancer and bacterial cases are increasing. Hence, new drugs to treat these diseases are paramount. Ferrocene-based hybrid compounds were synthesizedas potential cancer and bacteria therapeutics. The synthesized compounds were characterized via FTIR, NMR, and LC-MS and evaluated against different cancer cells and bacterial strains. Moreover, computational studies of these compounds were conducted using several silico tools. Among the synthesized compounds, hybrid 10 was the most promising compound, displaying promising anticancer activity with IC50 values between 42.42 and 45.37 and 50.64 and 73.37 µg/mL against HeLa and CHO cancer cells, respectively, with a selective index greater than one on HeLa cancer cells. Compounds 22–26 displayed promising antibacterial activity with a MIC value of 7.8125 µg/mL against most bacterial strains in vitro. The in silico results revealed that this compound has strong binding affinities for 4qtb, 3eqm, and 2w3l cervical cancer proteins, exhibiting binding energies of −7.3, −8.7, and 7.4 kcal/mol, respectively. Furthermore, hybrid 10 showed promising pharmacokinetics and drug-like properties, including high GI absorption, moderate water solubility, favoring the oral administration route, nontoxicity, and is a P-gp substrate. The findings obtained in this study illustrate that hybrid compounds are potential therapeutics that need to be explored. The compounds also contained functionalities relevant for incorporating into nanocarriers to improve their biological activities further. Therefore, further studies are recommended for the most effective compounds to reinforce these findings.Item Phytochemical characterization and cytotoxicity effects of Hyptis suaveolens collected in Nigeria(Elsevier B.V., 2025) Pearce, Keenau; Benjeddou, Mongi; Azeez, Olatunji GHyptis suaveleons (L.) Poit. belongs to the Laminaceae family. This weed, native to tropical America, has become widespread in tropical regions worldwide. In folk medicine, plant extracts are used in the treatment of wounds, peptic ulcers, respiratory tract infections, and skin diseases among others. Thus, the importance of assessing the toxicity of plants for any human use cannot be overstated. This study analyzed the phytochemical properties of the Nigerian H. suaveleons population and assessed its cytotoxicity. Six bioactive compounds were isolated from the acetone extract of the H. suaveolens plant using silica gel column chromatography, one of which is new from natural sources. The isolated compounds were identified as 14-O-acetyl suaveolol (C1), suaveolol (C2), suaveolic acid (C3), dehydroabietinol (C4), maniladiol (C5), and β-sitosterol (C6) based on 1D and 2D NMR spectroscopic data. The MTT assay was used to assess the cytotoxicity effects of these metabolites on HepG2, KMST-6, and HaCaT cells. Among them, C1 significantly reduced HepG2, KMST-6, and HaCaT cell viability with IC50 values of 35.97, 43.48, and 65.10 µg/ml, respectively. The study suggests C1 has promising anticancer properties, possibly due to the acetyl group on C-14, while C2, suaveolol, lacks this group, and did not yield any inhibitory activities. However, further research is needed to understand its mechanisms.Item Biomolecular affinities and cytotoxicity of copper(I) and silver(I) phosphine–pyridinyl complexes against CACO-2 and CASKI cell lines(John Wiley and Sons Inc, 2025) Meyer, Miche D; Sibuyi, Nicole R. S; Onani, Martin OA series of three copper (I) and three silver (I) complexes with the general formula [M L(PPh3)2]NO3, (M = Cu for complexes 1–3 and Ag for complexes 4–6) are synthesized by reacting copper(I) or silver(I)-nitrate and triphenylphosphine with the bidentate ligands, (E)-1-(pyridin-2-yl)-N-(o-tolyl)methanimine L1, (E)-N-isopropyl-1-(pyridine-2-yl)methanimine L2, or (E)-N-(2,6-dimethylphenyl)-1-(pyridine-2-yl)methanimine L3. The structures of these complexes are elucidated using a combination of NMR spectroscopy, FTIR, UV–visible, mass spectrometry, elemental analysis, and single-crystal X-ray diffraction. Structural analysis revealed that the Schiff bases coordinate to the metal centers in a bidentate fashion, with triphenylphosphine occupying the remaining coordination sites in complexes 1, 2, and 5. In contrast, in complexes 3, 4, and 6, one coordination site is occupied by a nitrate anion instead of triphenylphosphine. All six complexes exhibit a distorted tetrahedral geometry around the metal center, as confirmed by τ4 values ranging from 0.54 to 0.87. Binding studies with calf-thymus DNA demonstrated that complexes 1–6 interact via intercalation, with complex 5 exhibiting the highest binding constant. Furthermore, all complexes showed strong binding affinity toward bovine serum albumin. Cytotoxicity studies revealed significant cytotoxicity of complexes 1–6 against human colon adenocarcinoma (Caco-2) and human cervical epidermoid carcinoma (Caski) cell lines.Item Glucoselipid biosurfactant biosynthesis operon of rouxiella badensis DSM 100043T: screening, identification, and heterologous expression in escherichia coli(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Van Zyl Leonardo; Williams Wesley; Burger Anita; Harahap AndreRouxiella badensis DSM 100043T had been previously proven to produce a novel glucoselipid biosurfactant which has a very low critical micelle concentration (CMC) as well as very good stability against a wide range of pH, temperature, and salinity. In this study, we performed a function-based library screening from a R. badensis DSM 100043T genome library to identify responsible genes for biosynthesis of this glucoselipid. The identified open reading frames (ORFs) were cloned into several constructs in Escherichia coli for gene permutation analysis and the individual products were analyzed using high-performance thin-layer chromatography (HPTLC). Products of interest from positive expression strains were purified and analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and nuclear magnetic resonance (NMR) for further structure elucidation. Function-based screening of 5400 clones led to the identification of an operon containing three ORFs encoding acetyltransferase GlcA (ORF1), acyltransferase GlcB (ORF2), and phosphatase/HAD GlcC (ORF3). E. coli pCAT2, with all three ORFs, resulted in the production of identical R. badensis DSM 100043T glucosedilipid with Glu-C10:0-C12:1 as the main congener. ORF2-deletion strain E. coli pAFP1 primarily produced glucosemonolipids, with Glu-C10:0,3OH and Glu-C12:0 as the major congeners, predominantly esterified at the C-2 position of the glucose moiety. Furthermore, fed-batch bioreactor cultivation of E. coli pCAT2 using glucose as the carbon source yielded a maximum glucosedilipid titer of 2.34 g/L after 25 h of fermentation, which is 55-fold higher than that produced by batch cultivation of R. badensis DSM 100043T in the previous study.Item Delphinidin as an antineoplastic agent targeting androgen receptors(Wiley, 2025) Fadaka Adewale; Sibuyi Nicole; Alves LeandroResearchers globally have discovered that both red and green peppers are extremely rich in bioactive phytoconstituents with pharmacological properties. These metabolites are associated with critical physiological functions, including anticancer and health-promoting effects. However, due to insufficient clinical evidence or lack of standardized studies, there are no recommendations regarding their use and dosage. We investigated the potential therapeutic effects of Capsicum chinense flesh as an inhibitor of the androgen receptor through computational studies. To explore potential novel antitumor markers from Capsicum chinense flesh, we employed in silico docking, molecular dynamics simulations, and principal component analysis. Our results consistently demonstrated significant binding across all the receptor models tested, with the potential candidate (Delphinidin) emerged as a promising candidate. This study offers valuable insights into a robust pharmacological approach that could aid in identifying targets with investigative roles in tumor development and provide potential therapeutic options for managing tumors and associated challenges.Item The influence of season and land use on the occurrence of Metarhizium spp. in the Cape Peninsula region, South Africa(Taylor and Francis Ltd, 2025) Keyster, Marshall; Rhoda, Ilyaas; Prins, AlaricTo investigate the environmental relationships between the occurrence of Metarhizium spp., season and land use in the Cape Peninsula region, soil samples were collected from randomly selected sampling sites within nature reserves and agricultural farms. Soil samples from the same sites were collected in different seasons (winter and summer), and Metarhizium spp. were isolated from the soil samples by insect baiting and plating soil, and identified. Soil pH, texture, and nutrient contents of air-dried composite soil samples were analysed, and the seasonal rainfall data were recorded. The associations between the number of Metarhizium isolates with soil nutrient contents and seasonal rainfall were assessed. Farm sites had a significantly higher number of Metarhizium isolates (36 of 40 isolates collected) and higher concentrations of soil macronutrients, P, Ca and N (NO3), than in soil samples from the reserves, while higher average monthly rainfall in winter correlated with higher occurrences of Metarhizium isolates. In conclusion, wet, cold winters and vegetable farming favoured the higher occurrence of Metarhizium spp. in the Cape Peninsula region.Item Enhanced lactic acid production from potato peel waste via MnO2 nanoparticle-assisted simultaneous saccharification and fermentation: Process optimization and kinetic studies(Elsevier, 2025) Mambili-Mamboundou Hermane; Naidu Talia; Sanusi Isaac A.This study focused on optimizing lactic acid production from potato peel waste facilitated by MnO2 nanoparticle (NP). Also, kinetic analysis was conducted, utilizing the growth constant (Kg), logistic function, and modified Gompertz model to evaluate the process dynamics under optimized conditions and the control experiment (without NP). Under optimized conditions (0.034 wt% MnO2 NP, 6.02 pH, 34 ◦C temperature, and 14.90 wt% solid loading), notable increases in biomass and lactic acid concentrations of 2.85 g/L and 41.26 g/L were achieved representing a 1.16 and 1.42-fold increments respectively. In addition, the inclusion of MnO2 NP increased the maximum potential lactic acid concentration (Pm) and production rate, reaching 32.89 g/L and 1.51 g/L/h, respectively, compared to the control’s values of 29.80 g/L and 1.43 g/L/h. This study underscores the potential of MnO2 NP in boosting lactic acid bioprocessing and yield towards enhanced bioconversion of agricultural waste to useful products.Item UYSD: a novel data repository accessible via public website for worldwide population frequencies of Y-SNP haplogroups(Springer Nature, 2025) D’Amato, Maria E; Ralf, Arwin; Zandstra, DionFor decades, there has been scientific interest in the variation and geographic distribution of paternal lineages associated with the human Y chromosome. However, the relevant data have been dispersed across numerous publications, making it difficult to consolidate. Additionally, understanding the relationships between different variants, and the tools used to analyze them, have evolved over time, further complicating efforts to harmonize this information. The Universal Y-SNP Database (UYSD) marks a substantial advancement by providing a comprehensive and accessible platform for Y-SNP and haplogroup data from populations around the world. UYSD harmonizes diverse datasets into a unified repository, facilitating the exploration of global Y-chromosomal variation. The platform handles data generated with both high- and low-throughput technology and is compatible with the automated analysis software tool, Yleaf v3. Key functionalities include the ability to: i) visualize haplogroup distributions on an interactive world map, ii) estimate haplogroup frequencies in geographic regions with sparse data through interpolation, and iii) display detailed phylogenetic trees of Y-chromosomal haplogroups. Currently, UYSD encompasses data from over 6,600 males across 27 populations. This dataset largely aligns with known global Y-haplogroup patterns, but also reveals unexplored finer-scale geographic variations. While the present dataset is largely European-centered, UYSD is designed for ongoing expansion by the scientific community, aiming to include more global data and higher-resolution population sequencing data. The platform thus offers valuable insights into human genetic diversity and migration patterns, serving several fields of research such as: human population genetics, genetic anthropology, ancient DNA analysis and forensic genetics.Item Fimsbactin siderophores from a South African marine sponge symbiont, marinomonas sp. PE14-40(John Wiley and Sons Ltd, 2025) Ikegwuoha, Nompumelelo Philile Praiseworth; Hanekom, Thea; Booysen, Elzaan; Jason, Corbyn; van Zyl, Leonardo Joaquim; Trindade, MarlaLow iron levels in marine habitats necessitate the production of structurally diverse siderophores by many marine bacterial species for iron acquisition. Siderophores exhibit bioactivities ranging from chelation for iron reduction in hemochromatosis sufferers to antimicrobial activity either in their own right or when coupled to known antibiotics for targeted delivery or for molecular imaging. Thus, marine environments are a sought-after resource for novel siderophores that could have pharmaceutical or industrial application. The fimsbactins A-F (1–6) are mixed catechol-hydroxamate siderophores that have only been reported to be produced by Acinetobacter species with the fimsbactin biosynthetic gene clusters (BGCs) widespread among species within this genus. Here, we identified a putative fimsbactin BGC from an uncharacterized marine isolate, Marinomonas sp. PE14-40. Not only was the gene synteny not conserved when comparing the pathway from Marinomonas sp. PE14-40 to the fimsbactin BGC from Acinetobacter sp., but five of the core biosynthetic genes found in the canonical fimsbactin BGC are located elsewhere on the genome and do not form part of the core cluster in Marinomonas sp. PE14-40, with four of these, fbsBCDL, colocalized. Through ESI-MS/MS analysis of extracts from Marinomonas sp. PE14-40, the known fimsbactin analogues 1 and 6 were identified, as well as two new fimsbactin analogues, 7 and 8, containing a previously unreported L-lysine-derived hydroxamate moiety, N1-acetyl-N1-hydroxycadaverine. Feeding experiments using stable isotope-label L-lysine provided further evidence of the N1-acetyl-N1-hydroxycadaverine moiety in 7 and 8. The study demonstrates functional conservation in seemingly disparate biosynthetic pathways and enzyme promiscuity's role in producing structurally diverse compounds.Item Effects of acidic and alkaline electrolyzed water treatments on the volatilomics and proteomics changes in fresh-cut apple during storage(Royal Society of Chemistry, 2025) Mohamed, Gadija; Caleb, Oluwafemi James; Belay, Zinash A.Electrolyzed water (EW) has shown potential to decontaminate and maintain the quality of fresh-cut apple; however, the underlying response of the product to this treatment remains unclear. Thus, this study aims to identify the possible quality regulation mechanisms of acidic electrolyzed water (AEW) and alkaline electrolyzed water (ALEW) treatments on fresh-cut ‘Granny Smith’ apples via volatile organic compound (VOC) and qualitative proteomics analysis during storage at 2 °C for 10 days. The results identified 43 VOCs, including 10 esters, 9 alcohols, 9 alkanes, 8 carboxylic acids, 6 ketones, and 1 aldehyde. The distribution of VOCs was significantly affected by the pretreatment conditions; fresh-cut apple treated with AEW was characterised by the highest number of esters, alcohols, and carboxylic acids, whereas samples treated with ALEW exhibited predominantly carboxylic acids, alcohols, and alkanes in comparison to control (untreated) samples. Ethyl dodecanoate, which was identified only in the ALEW samples on each sampling day, had the highest concentration among all the individual VOCs. The proteomics results showed that a total of 3434, 3401, and 3313 proteins were identified on day 3, 6, and 10, respectively, across all samples. Until day 6 of storage, no significant differences were observed among the samples. Notably, on day 6, “M16C_associated domain-containing protein” was shown to be unique to the control samples. KH type-2 domain-containing protein, methylenetetrahydrofolate reductase (MTHFR), and 1,4-alpha-glucan branching enzyme were unique proteins identified after AEW treatment at day 6 and 10 of storage. No unique protein was identified for the ALEW samples. These results provide the first report of the proteomic and volatilomic changes associated with EW-treated fresh-cut apple during storage.Item Sex and environment shape cochlear sensitivity in human populations worldwide(Elsevier B.V, 2025) Croze, Myriam; Balaresque, Patricia; Delmotte, SébastienHearing remains an underexplored aspect of human evolution. While the growing prevalence of hearing issues worldwide highlights the need to investigate factors beyond age, ototoxic substances, and recreational noise— factors affecting only a subset of the population —the role of environmental influences remains relatively unaddressed. In contrast, hearing and vocalizations have been extensively studied in many vertebrates through the Acoustic Adaptation Hypothesis, which suggests that acoustic communication adapts to the structure of the immediate environment. To explore how the environment shapes the ear’s ability to process sound, studying the cochlea is essential since it is responsible for capturing, amplifying, and converting sound waves into electrical signals. Cochlear sensitivity can be measured using Transient-Evoked Otoacoustic Emissions (TEOAE), which assess the cochlea’s ability to produce and transmit an acoustic response after sound stimulation. By analyzing TEOAE profiles, we gain valuable insights into how the cochlea responds to external auditory stimuli. We evaluated the influence of both endogenous (age, sex, ear side) and exogenous factors (ethnicity, environment, language) on cochlear sensitivity by collecting TEOAE data from 448 healthy individuals across 13 global populations in Ecuador, England, Gabon, South Africa, and Uzbekistan, living in diverse environments. For each individual, we derived six acoustic metrics from these TEOAE profiles to characterize the amplitude and frequency spectrum of cochlear sensitivity. Our results show that amplitude is primarily influenced by sex (up to 2 dB) and environment (up to 3.6 dB), followed by age and ear side. The frequency spectrum is determined exclusively by exogenous factors, with environment— particularly altitude, and urban versus rural settings —being the most significant. These findings challenge existing assumptions and highlight the need to consider both biological and environmental factors when studying auditory processes.Item Enhancing Fusarium oxysporum tolerance in Phaseolus vulgaris: Isolation and characterization of bacterial endophytes(Elsevier B.V., 2025) Holman, Darin Edward; Keyster, Marshall; Klein, Ashwil; Daniel, Augustine InnalegwuResearch surrounding microbial biocontrol has garnered considerable attention in the last few years due to the numerous advantages as opposed to synthetic agrochemicals. Phaseolus vulgaris is an important legume that is cultivated worldwide, especially in third-world countries. The growth and yield of this crop are often severely limited by the pathogen Fusarium oxysporum. The aim of the study is to investigate the biological control potential of six plant bacterial isolates against F. oxysporum, both in vitro and in vivo. Six bacterial endophytes were isolated from Ledebouria ovatifolia leaves (E1), Solanum dulcamara leaves (E2), Cortalaria retusa leaves (E3), Euphorbia prostrata roots (R1), Solanum nigrum roots (R2), and Helichrysum splendidum roots (R3). These isolates were characterized for any biocontrol activity they might have against F. oxysporum PPR1. Isolation, characterization, identification, and biocontrol antagonistic assays were carried out in vitro against F. oxysporum, following standard protocols. The isolates were identified using 16S rRNA gene PCR sequencing. A phylogenetic analysis indicated that the leaf isolates displayed a close relationship with Bacillus altitudinis (E1), Streptomyces bikiensis strain SBM (E2), and Pseudomonas rhodesiae (E3). Root isolates displayed a close relationship with Enterobacter kobei (R1), Enterobacter sp. (R2), and Pseudomonas sp. strain (R3). Five out of the six isolates exhibited catalase activity and zinc solubilization activity, whereas all isolates exhibited siderophore production activity. Most of the tested isolates were able to produce the extracellular hydrolytic enzymes protease and amylase. The isolates exhibiting the highest hydrolytic enzyme activities were able to significantly inhibit F. oxysporum growth in vitro. E. kobei exhibited the most promising plant growth-promoting activity, hydrolytic enzyme activity, F. oxysporum antagonism, and increased seedling growth of P. vulgaris. These results suggest that E. kobei represents a good biocontrol candidate against F. oxysporum. The ability of this bacterial isolate to colonize and its promising biological activities suggest it has enormous potential to be used as both a biopesticide and plant growth stimulator.Item Interference of a phytoconstituent from Nymphaea lotus-derived ligand N-acetyl glucosamine with signaling receptors in diabetes mellitus development: a targeted computational analysis(Elsevier B.V., 2025) Fadaka, Adewale Oluwaseun; Onikanni, Sunday Amos; Dao, Tran Nhat-PhongDiabetes mellitus is a world-wide health concern with several millions affected in all ages. Computer-aided drug design (CADD) is a powerful tool that has revolutionized the process of discovering and developing new drugs. It provides innovative methods that can speed up drug discovery and lower costs thereby results to increase enthusiasm at developing instinctive antidiabetic agents as alternatives for managing diabetes. Nymphaea lotus, a plant with medicinal properties known for its anti-diabetic effects, contains bioactive components like N-acetyl glucosamine. An in silico study was conducted to investigate its potential in targeting proteins related to diabetes. Molecular docking studies, toxicity prediction, examination of drug depiction, and Molecular Dynamics Simulation (MDs) of the ligands with the identified receptor target were conducted using the Schrödinger platform. The receptor-ligand complex of Nymphaea lotus was compared with known inhibitors. Molecular dynamics simulation, principal component analysis, and free energy landscape analysis showed that the binding affinity of the Nymphaea lotus complex was higher than that of reference ligands. This suggests that Nymphaea lotus and its bioactive compounds have promising medicinal value for managing type 2 diabetes, warranting further research into their therapeutic potentialItem Potential matrix metalloproteinase 2 and 9 inhibitors identified from Ehretia species for the treatment of chronic wounds - Computational drug discovery(Elsevier Ltd, 2025) Oselusi, Samson; Martin, Darius; Meyer, Mervin; Madiehe, Abram; Sibuyi, NicoleMatrix metalloproteinases (MMPs) serve as prognostic factors in several pathophysiological conditions, including chronic wounds. Therefore, they are considered important therapeutic targets in the intervention and treatment of these conditions. In this study, computational tools such as molecular docking and molecular dynamics simulations were used to gain insight into protein‒ligand interactions and determine the free binding energy between Ehretia species phytoconstituents and gelatinases (MMP2 and MMP9). A total of 74 phytoconstituents from Ehretia species were compiled from the literature, and 46 of these compounds were identified as potential inhibitors of at least one type of MMP. Molecular docking revealed that lithospermic acid B, rosmarinic acid, and danshensu had stronger binding affinities against the two enzymes than the reference ligands. Furthermore, (9S, 10E, 12Z, 15Z)-9-hydroxy-10,12,15-octadecatrienoic (∗-octadecatrienoic) had a higher binding energy for MMP2, whereas caffeic anhydride and caffeic acid established stronger binding energy with MMP9 than the reference ligand. These complexes also demonstrated relatively stable, favourable, and comparable conformational changes with those of unbound proteins at 500 ns. The free energy decomposition results further provide detailed insights into the contributions of active site residues and different types of interactions to the overall binding free energy. Finally, most of the hit phytoconstituents (rosmarinic acid, caffeic anhydride, caffeic acid, and danshensu) had good physicochemical, drug-likeness, and pharmacokinetic properties. Collectively, our findings showed that phytoconstituents from Ehretia species could be beneficial in the search for novel MMP inhibitors as therapeutic agents for the treatment of chronic wounds.Item Nano-based co-valorization, detoxification, and fermentation of potato waste and black liquor for bioethanol production(Springer Science and Business Media Deutschland GmbH, 2025) Sewsynker-Sukai, Yeshona; Zondi, Amanda; Sanusi, IsaacBlack liquor from paper and pulp mills is an underutilized waste resource. Black liquor can be valorized due to its useful chemical fractions and water component that can be employed for the pretreatment and biochemical conversion of starchy potato waste (SPW) into bioethanol. Hence, in this study, the simultaneous co-valorization, detoxification, and fermentation of SPW and black liquor (without the addition of freshwater and chemicals) were optimized for bioethanol production. The scanning electron microscopy (SEM) and the Fourier-transform infrared (FTIR) clearly confirmed the distortion of SPW inherent structure for the recovery of useful carbohydrates. A significant reduction in process inhibitors (furfural = 1.26-fold, hydroxymethylfurfural (HMF) = 5.68-fold, and phenols = 1.03-fold) was observed with CuO NP inclusion. The response surface methodology (RSM) model of the bioethanol production showed a high coefficient of determination (R2) value of 0.98. The optimized process with CuO NP inclusion displayed a biomass and bioethanol concentrations of 2.12 g/L and 21.37 g/L corresponding to 1.74- and 1.68-fold improvement over the control respectively. In addition, the kinetic data showed that the incorporation of CuO nanoparticle (NP) significantly improved (1.86-fold) the potential maximum bioethanol concentration (Pm) (20.21 g/L) compared to the control experiment (10.86 g/L). The study demonstrates a 100% freshwater conservation approach for improved sugar recovery, remarkable inhibitor removal, and bioethanol production from pretreated SPW towards reduced biofuel production cost, waste management, and green environment sustainability.Item Effects of acidic and alkaline electrolyzed water treatments on the volatilomics and proteomics changes in fresh-cut apple during storage(Royal Society of Chemistry, 2025) Caleb, Oluwafemi James; Belay, Zinash A.; Nkomo, MbukeniElectrolyzed water (EW) has shown potential to decontaminate and maintain the quality of fresh-cut apple; however, the underlying response of the product to this treatment remains unclear. Thus, this study aims to identify the possible quality regulation mechanisms of acidic electrolyzed water (AEW) and alkaline electrolyzed water (ALEW) treatments on fresh-cut ‘Granny Smith’ apples via volatile organic compound (VOC) and qualitative proteomics analysis during storage at 2 °C for 10 days. The results identified 43 VOCs, including 10 esters, 9 alcohols, 9 alkanes, 8 carboxylic acids, 6 ketones, and 1 aldehyde. The distribution of VOCs was significantly affected by the pretreatment conditions; fresh-cut apple treated with AEW was characterised by the highest number of esters, alcohols, and carboxylic acids, whereas samples treated with ALEW exhibited predominantly carboxylic acids, alcohols, and alkanes in comparison to control (untreated) samples. Ethyl dodecanoate, which was identified only in the ALEW samples on each sampling day, had the highest concentration among all the individual VOCs. The proteomics results showed that a total of 3434, 3401, and 3313 proteins were identified on day 3, 6, and 10, respectively, across all samples. Until day 6 of storage, no significant differences were observed among the samples. Notably, on day 6, “M16C_associated domain-containing protein” was shown to be unique to the control samples. KH type-2 domain-containing protein, methylenetetrahydrofolate reductase (MTHFR), and 1,4-alpha-glucan branching enzyme were unique proteins identified after AEW treatment at day 6 and 10 of storage. No unique protein was identified for the ALEW samples. These results provide the first report of the proteomic and volatilomic changes associated with EW-treated fresh-cut apple during storage. Data are available via ProteomeXchange with identifier PXD056621. © 2025 RSC.Item Survey and Identification of Fusarium Head Blight Pathogens of Wheat in the Western Cape Region of South Africa(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Ali, Al-Hashimi,; Augustine Innalegwu, Daniel; Omolola, AinaFusarium head blight (FHB) is a major disease affecting wheat production worldwide, caused by multiple Fusarium species. In this study, seven Fusarium strains were isolated from wheat fields across the Western Cape region of South Africa and identified through phylogenetic analysis. The strains were classified into three species complexes: the Fusarium graminearum species complex (FGSC), Fusarium incarnatum-equiseti species complex (FIESC), and Fusarium tricinctum species complex (FTSC). Disease severity was highest in the South coast regions of Swellendam (42.73%) and Caledon (38.00%), with the dough stage of wheat development showing the highest disease rate (0.3 in Swellendam and Caledon). The phylogenetic analysis showed distinct clustering of these isolates with known species from the NCBI database, confirming their classification. F. ipomoeae was uniquely found in Swellendam and Caledon, while F. tricinctum occurred only in Klipheuwel and Caledon, highlighting geographical variation in species distribution. Mycotoxin profiling revealed that F. culmorum and F. pseudograminearum produced zearalenone, F. culmorum and F. tricinctum produced 15-acetyl-deoxynivalenol (15-ADON), while F. pseudograminearum produced nivalenol (NIV). These findings provide significant insights into the distribution of Fusarium species and their associated trichothecene chemotypes in the Western Cape, which is crucial for developing effective FHB management strategies and ensuring food security and safety. © 2025 by the authors.Item Effects of ZnO nanoparticles concentration on the morphology and textural properties of ZnO/NiFe2O4 nanocomposite(Elsevier B.V., 2025) Daniel, Augustine Innalegwu; Keyster, Marshall; Tijani, Jimoh OladejoThe aim of this study is to synthesize and characterize ZnO and NiFe2O4 nanoparticles via green route and co-precipitation of ZnO/NiFe2O4. X-ray diffraction (XRD) data show no extra diffraction peaks belonging to other phases except wurtzite. High resolution transmission electron microscopy (HRTEM) images showed that the average interplanar distance of wurtzite phase at 3, 5, and 7 % dopant concentration were about 0.28, 0.44 and 0.33 nm respectively. X-ray photoelectron spectroscopy (XPS) results show difference in binding energies of the elements present in different concentration of the dopants. Electron Energy Loss Spectroscopy (EELS) spectra show similarities in the shape of Zn, Fe and Ni from zero loss, low loss and core loss region with a little shift in energy. All the elements exhibit multiple oxidation state; +2 and +3 for Fe and +1 and +2 for Zn and Ni. Brunauer-Emmett-Teller (BET) plot shows that ZnO belongs to the type II isotherm curve while NiFe2O4 and 3, 5 and 7 % ZnO/NiFe2O4 all belong to type IV isotherm curve indicating ZnO as macroporous while NiFe2O4 and different dopant concentration of ZnO/NiFe2O4 are mesoporous. The study shows the complete synthesis of ternary ZnO/NiFe2O4 nanocomposites using green synthesis and sol-gel approach. • Green synthesis of ZnO and NiFe2O4 using leaf extract of Anacardium occidentale • Co-precipitation method at different concentration of ZnO and NiFe2O4 for the synthesis of ZnO/NiFe2O4. • Nanocomposites was characterized using different analytical tools