Graphenated organic nanoparticles immunosensors for the detection of TB biomarkers
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Date
2017
Authors
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Journal ISSN
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Publisher
University of the Western Cape
Abstract
Pulmonary Tuberculosis (TB) a disease second to HIV/AIDS is a global health problem that
arises in two states; as an active state and as a latent state. Diagnosis of active TB is tedious
and requires expensive procedures since there is no recognizable method for the sole detection
of active TB. The current diagnosis consists of chest X-rays and multiple sputum cultures used
for acid-fast bacilli detection. The TB diagnosis of children is particularly difficult which
further complicates the diagnosis. Thus, rapid identification of this pathogen is important for
the treatment and control of this infection to allow effective and timely therapy. In an effort to
solve this issue, this study reports the development of immunosensors constructed with
electroactive layers of amino groups functionalized graphene oxide (GO) doped respectively
with green synthesized zinc oxide (ZnO NPs) nanoparticles and silver (Ag NPs) nanoparticles
on glassy carbon electrodes. The surface morphology of GO, ZnO NPs, Ag NPs and their
composites was revealed by employing High-Resolution Transmission Electron Microscopy
(HR-TEM) and High-Resolution Scanning Electron Microscopy (HR-SEM) while the
composition and structure of these materials were studied using Fourier Transform Infra-Red
Spectroscopy (FTIR). The resultant graphene oxide-metallic composites were covalently
attached with CFP-10 and/or ESAT-6 antibodies to achieve the electrochemical detection. The
immunosensor was then used for the impedimetric and amperometric detection of anti-CFP-10
and/or anti-ESAT-6 antigens in standard solutions.
Description
Magister Scientiae - MSc (Chemistry)
Keywords
Tuberculosis, Graphene oxide, Zinc oxide nanoparticles, Silver Nanoparticles, Composites, Nanoalloys, Antibodies, Immunosensors