A proteomic analysis of drought and salt stress responsive proteins of different sorghum varieties

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dc.contributor.advisorRees, Jasper
dc.contributor.authorNgara, Rudo
dc.date.accessioned2014-01-22T14:09:15Z
dc.date.accessioned2024-05-09T08:19:14Z
dc.date.available2012/04/16 14:29
dc.date.available2012/04/16
dc.date.available2014-01-22T14:09:15Z
dc.date.available2024-05-09T08:19:14Z
dc.date.issued2009
dc.descriptionPhilosophiae Doctor - PhDen_US
dc.description.abstractThis study reports on a proteomic analysis of sorghum proteomes in response to salt and hyperosmotic stresses. Two-dimensional gel electrophoresis (2DE) in combination with mass spectrometry (MS) was used to separate, visualise and identify sorghum proteins using both sorghum cell suspension cultures and whole plants. The sorghum cell suspension culture system was used as a source of culture filtrate (CF) proteins. Of the 25 visualised CBB stained CF spots, 15 abundant and well-resolved spots were selected for identification using a combination of MALDI-TOF and MALDI-TOFTOF MS, and database searching. Of these spots, 14 were positively identified as peroxidases, germin proteins, oxalate oxidases and alpha-galactosidases with known functions in signalling processes, defense mechanisms and cell wall metabolism.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/13503
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectSorghum proteomicsen_US
dc.subjectCerealsen_US
dc.subjectDrought and salinity stressesen_US
dc.subjectSorghum cell suspension culturesen_US
dc.subjectSecretomeen_US
dc.subject2D SDS-PAGEen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectMALDI-TOF-TOF MSen_US
dc.subjectProtein identificationen_US
dc.subjectProteome reference mapsen_US
dc.titleA proteomic analysis of drought and salt stress responsive proteins of different sorghum varietiesen_US
dc.typeThesisen_US

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Philosophiae Doctor - PhD (Biotechnology)