Impedimetric microcystin-lr aptasensor prepared with sulfonated poly(2,5-dimethoxyaniline)–silver nanocomposite
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
MPDI
Abstract
This paper presents a novel impedimetric aptasensor for cyanobacterial microcystin-LR
(L, L-leucine; R, L-arginine) (MC-LR) containing a 50
thiolated 60-mer DNA aptamer (i.e., 50
-SH-
(CH2
)6GGCGCCAAACAGGACCACCATGACAATTACCCATACCACCTCATTATGCCCCATCT CCGC3
0
). A nanocomposite electrode platform comprising biocompatible poly(2,5-dimethoxyaniline) (PDMA)-
poly(vinylsulfonate) (PVS) and silver nanoparticle (Ag0
) on a glassy carbon electrode (GCE), i.e.,
(GCE/PDMA–PVS–Ag0
) was used in the biosensor development. Small-angle X-ray scattering (SAXS)
spectroscopic analysis revealed that the PDMA–PVS–Ag0 nanocomposites were polydispersed and
contained embedded Ag0
. Electrochemical impedance spectroscopy (EIS) responses of the aptasensor
gave a dynamic linear range (DLR) and limit of detection (LOD) values of 0.01–0.1 ng L−1 MC-LR and
0.003 ng L−1 MC-LR, respectively. The cross-reactivity studies, which was validated with enzyme-linked
immunosorbent assay (ELISA), showed that the aptasensor possesses excellent selectivity for MC-LR.
Description
Keywords
Electrochemical aptasensor, Silver nanoparticles, Chemistry, Electrochromic devices
Citation
Bilibana, M. P. et al. (2021). Impedimetric microcystin-lr aptasensor prepared with sulfonated poly(2,5-dimethoxyaniline)–silver nanocomposite. Processes, 9(1), 179. https://doi.org/10.3390/pr9010179