Impedimetric microcystin-lr aptasensor prepared with sulfonated poly(2,5-dimethoxyaniline)–silver nanocomposite

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dc.contributor.authorBilibana, Mawethu Pascoe
dc.contributor.authorFeleni, Usisipho
dc.contributor.authorWilliams, Avril Rae
dc.date.accessioned2022-06-14T08:18:42Z
dc.date.available2022-06-14T08:18:42Z
dc.date.issued2021
dc.description.abstractThis paper presents a novel impedimetric aptasensor for cyanobacterial microcystin-LR (L, L-leucine; R, L-arginine) (MC-LR) containing a 50 thiolated 60-mer DNA aptamer (i.e., 50 -SH- (CH2 )6GGCGCCAAACAGGACCACCATGACAATTACCCATACCACCTCATTATGCCCCATCT CCGC3 0 ). A nanocomposite electrode platform comprising biocompatible poly(2,5-dimethoxyaniline) (PDMA)- poly(vinylsulfonate) (PVS) and silver nanoparticle (Ag0 ) on a glassy carbon electrode (GCE), i.e., (GCE/PDMA–PVS–Ag0 ) was used in the biosensor development. Small-angle X-ray scattering (SAXS) spectroscopic analysis revealed that the PDMA–PVS–Ag0 nanocomposites were polydispersed and contained embedded Ag0 . Electrochemical impedance spectroscopy (EIS) responses of the aptasensor gave a dynamic linear range (DLR) and limit of detection (LOD) values of 0.01–0.1 ng L−1 MC-LR and 0.003 ng L−1 MC-LR, respectively. The cross-reactivity studies, which was validated with enzyme-linked immunosorbent assay (ELISA), showed that the aptasensor possesses excellent selectivity for MC-LR.en_US
dc.identifier.citationBilibana, M. P. et al. (2021). Impedimetric microcystin-lr aptasensor prepared with sulfonated poly(2,5-dimethoxyaniline)–silver nanocomposite. Processes, 9(1), 179. https://doi.org/10.3390/pr9010179en_US
dc.identifier.issn2227-9717
dc.identifier.urihttps://doi.org/10.3390/pr9010179
dc.identifier.urihttp://hdl.handle.net/10566/7518
dc.language.isoenen_US
dc.publisherMPDIen_US
dc.subjectElectrochemical aptasensoren_US
dc.subjectSilver nanoparticlesen_US
dc.subjectChemistryen_US
dc.subjectElectrochromic devicesen_US
dc.titleImpedimetric microcystin-lr aptasensor prepared with sulfonated poly(2,5-dimethoxyaniline)–silver nanocompositeen_US
dc.typeArticleen_US

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