Browsing by Author "Saibu, Gbemisola M."

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    In-vitro cytotoxicity, anti-bacterial and anti-inflammatory activities of ecklonia radiataand jania verrucosafrom Eastern Cape, South Africa
    (University of Benin, 2022) Saibu, Gbemisola M.; Vincent, Nakin M.; Olawale, Fadaka O.
    Seaweeds are marine floras constituting in part to over 90% of the oceanic biomass. Despite the enrichments of seaweeds, their potentials are largely unexplored. Hence, this study evaluated the phytochemical screening, antimicrobial, anti-inflammatory and cytotoxicity activities of methanolic extracts of Ecklonia radiata and Jania verrucosa. E. radiata and J. verrucosa were collected from sites on the rocky shores of the Mbashe and Port St. John rivers on the Eastern Cape coast of South Africa during spring low tides. Qualitative phytochemical screening was carried out using standard protocol. The assessment of anti-inflammatory activity was done by inhibition of albumin denaturation assay and antimicrobial analysis was performed against six different pathogenic clinical isolates. The cytotoxicity effect of the methanolic extracts were further investigated on MDA-MB-231, HepG2, CaCo-2 and KMST-6 by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay (MTT assay). Phytochemical analysis revealed the presence of tannins, phenols, glycosides, and alkaloid for both extracts. The methanolic extracts of both seed weeds also possess anti-inflammatory activity by inhibiting albumin denaturation.
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    Synthesis, computational and biological studies of alkyltin(IV) N-methyl-N-hydroxyethyl dithiocarbamate complexes
    (Elsevier, 2021) Adeyemi, Jerry O.; Saibu, Gbemisola M.; Fadaka, Adewale O.
    Methyltin(IV) of butyltin(IV)–N-hydroxyethyl dithiocarbamate complexes, represented as [(CH3)2Sn(L(OH))2] and [(C4H9)2Sn(L(OH))2] respectively were synthesized and characterized using spectroscopic techniques (1 H, 13C and 119Sn NMR) and elemental analysis. Both infrared and NMR data showed that, the complexes were formed via two sulphur atoms of the dithiocarbamate group. This mode of coordination was further supported by the DFT calculation, which suggested the formation of a distorted octahedral geometry around the tin atom. The complexes were screened for their antioxidant, cytotoxicity and anti-inflammatory properties. Four different assays including DPPH, nitric oxide, reducing power and hydrogen peroxides were used for the antioxidant studies, while an in vitro anti-inflammatory study was done using albumin denaturation assay. The complexes showed good antioxidant activity, especially in the DPPH assay. Butyltin(IV)–N-hydroxyethyl dithiocarbamate showed better cytotoxicity activity compared to methyltin(IV)–N-hydroxyethyl dithiocarbamate in the selected cell lines, which included KMST-6, Caco-2 and A549 cell lines. The anti-inflammatory activities revealed that the two complexes have useful activities better than diclofenac used as control drug.