Application on gold nanoparticles-dotted 4-nitrophenylazo graphene in a label-free impedimetric deoxynivalenol immunosensor
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Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
In this paper, we report a new concept to construct a label-free electrochemical
inhibition-based immunosensor for the detection of the mycotoxin deoxynivalenol (DON)
in cereal samples. The electrochemical impedance spectroscopy of tris(bipyridine)
ruthenium (II) chloride was used as a marker enhanced with gold nanoparticles-dotted
4-nitrophenylazo functionalized graphene (AuNp/G/PhNO2) nanocatalyst mediated in
Nafion on a glassy carbon electrode. Under the optimized conditions, the formation of
immunocomplexes inhibited electron flow and increased the charge transfer resistance of
the sensing interface linearly. The change in impedance was proportional to DON
concentrations in the range of 6–30 ng/mL with a sensitivity and detection limit of
32.14 ΩL/ng and 0.3 µg/mL, respectively, which compares favorably with the ELISA
result. The proposed sensor had a stability of 80.3%, good precision and selectivity in
DON standard solution containing different interfering agents, indicating promising
application prospect for this strategy in designing impedimetric, electrochemiluminescent,
voltammetric or amperometric sensors.
Description
Keywords
Deoxynivalenol, Mycotoxin, Graphene, Immunosensor, Graphene oxide, Nafion
Citation
Sunday, C. E. (2015). Application on gold nanoparticles-dotted 4-nitrophenylazo graphene in a label-free impedimetric deoxynivalenol immunosensor. Sensors, 15:3854-3871