GlobalFiler® express DNA amplification kit in South Africa: Extracting the past from the present

Simple item page
dc.contributor.authorRistow, Peter Gustav
dc.contributor.authorCloete, Kevin Wesley
dc.contributor.authorD’Amato, Maria Eugenia
dc.date.accessioned2023-02-06T09:35:46Z
dc.date.available2023-02-06T09:35:46Z
dc.date.issued2016
dc.description.abstractIn this study, the GlobalFiler1 Express amplification kit was evaluated for forensic use in 541 South African individuals belonging to the Afrikaaner, amaXhosa,1 amaZulu,1 Asian Indian and Coloured population groups. Allelic frequencies, genetic diversity parameters and forensic informative metrics were calculated for each population. A total of 301 alleles were observed ranging between 5 and 44.2 repeat units, 43 were rarely observed partial repeats and seven were novel. The combined match probability (CMP) ranged from 2.21 10 26 (Coloured) to 5.21 10 25 (AmaZulu), and the combined power of exclusion (CPE) 0.9999999978 (Afrikaaner) to 0.99999999979 (AmaZulu) respectively. No significant departures from Hardy-Weinberg equilibrium (HWE) were observed after Bonferroni correction. Strong evidence of genetic structure was detected using the coancestry coefficient u, Analysis of Molecular Variance (AMOVA) and an unsupervised Bayesian clustering method (STRUCTURE). The efficiency of assignment of individuals to population groups was evaluated by applying likelihood ratios with WHICHRUN, and the individual ancestral membership probabilities inferred by STRUCTURE. Likelihood ratios performed the best in the assignment of individuals to population groups. Signs of positive selection were detected for TH01 and D13S317 and purifying/balancing selection for locus SE33. These three loci also displayed the largest informativeness for assignment (In) values.en_US
dc.identifier.citationRistow, P. G. et al. (2016). GlobalFiler® express DNA amplification kit in South Africa: Extracting the past from the present. Forensic Science International: Genetics, 24, 194-201. https://doi.org/10.1016/j.fsigen.2016.07.007en_US
dc.identifier.issn1872-4973
dc.identifier.urihttps://doi.org/10.1016/j.fsigen.2016.07.007
dc.identifier.urihttp://hdl.handle.net/10566/8356
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectForensicsen_US
dc.subjectBiotechnologyen_US
dc.subjectRaceen_US
dc.subjectSouth Africaen_US
dc.subjectGlobalFileren_US
dc.titleGlobalFiler® express DNA amplification kit in South Africa: Extracting the past from the presenten_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ristow_globalfiler express dna amplification kit_2016.pdf
Size:
1.93 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles (Biotechnology)