GlobalFiler® express DNA amplification kit in South Africa: Extracting the past from the present
Loading...
Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
In this study, the GlobalFiler1 Express amplification kit was evaluated for forensic use in 541 South
African individuals belonging to the Afrikaaner, amaXhosa,1 amaZulu,1 Asian Indian and Coloured
population groups.
Allelic frequencies, genetic diversity parameters and forensic informative metrics were calculated for
each population. A total of 301 alleles were observed ranging between 5 and 44.2 repeat units, 43 were
rarely observed partial repeats and seven were novel. The combined match probability (CMP) ranged
from 2.21 10 26 (Coloured) to 5.21 10 25 (AmaZulu), and the combined power of exclusion (CPE)
0.9999999978 (Afrikaaner) to 0.99999999979 (AmaZulu) respectively. No significant departures from
Hardy-Weinberg equilibrium (HWE) were observed after Bonferroni correction.
Strong evidence of genetic structure was detected using the coancestry coefficient u, Analysis of
Molecular Variance (AMOVA) and an unsupervised Bayesian clustering method (STRUCTURE). The
efficiency of assignment of individuals to population groups was evaluated by applying likelihood ratios
with WHICHRUN, and the individual ancestral membership probabilities inferred by STRUCTURE.
Likelihood ratios performed the best in the assignment of individuals to population groups. Signs of
positive selection were detected for TH01 and D13S317 and purifying/balancing selection for locus SE33.
These three loci also displayed the largest informativeness for assignment (In) values.
Description
Keywords
Forensics, Biotechnology, Race, South Africa, GlobalFiler
Citation
Ristow, P. G. et al. (2016). GlobalFiler® express DNA amplification kit in South Africa: Extracting the past from the present. Forensic Science International: Genetics, 24, 194-201. https://doi.org/10.1016/j.fsigen.2016.07.007