The characterization and phylogenetic relationship of the trichoplusia ni single capsid nuclear polyhedrosis virus polyhedrin gene

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dc.contributor.authorFielding, Burtram C.
dc.contributor.authorDavison, Sean
dc.date.accessioned2013-11-28T07:48:23Z
dc.date.available2013-11-28T07:48:23Z
dc.date.issued1999
dc.description.abstractThe polyhedrin gene (polh) was identified from the Trichoplusia ni (Tni) single capsid nuclear polyhedrosis virus (SNPV). An EcoRI fragment containing the truncated polyhedrin gene was detected by hybridization with an AcMNPV expression vector probe; the remaining portion of the gene was amplified by reverse PCR. An open reading frame (ORF) of 741 nucleotides (nt), encoding a putative protein of 246 amino acids (a.a) with Mr 28,780 Da was identified. The 50-noncoding region contained the putative late (TAAG) transcription initiation motif. The 30 end, downstream of the translation stop codon, lacked an obvious putative poly (A) signal. Nucleotide and amino acid homology are greater than 80% to that of Mamestra brassicae polyhedrin sequences.Results suggest that T. niSNPV is a member of the group II nuclear polyhedrosis viruses.en_US
dc.description.accreditationISIen_US
dc.identifier.citationFielding, B.C. & Davison, S. (1999). The characterization and phylogenetic relationship of the trichoplusia ni single capsid nuclear polyhedrosis virus polyhedrin gene. Virus Genes, 19(1): 67-72en_US
dc.identifier.issn0920-8569
dc.identifier.urihttp://hdl.handle.net/10566/879
dc.identifier.urihttp://dx.doi.org/10.1023/A:1008192723971
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherKluwer Academic Publishersen_US
dc.rightsThis is the post-print version of the article published available online at http://dx.doi.org/10.1023/A:1008192723971
dc.source.urihttp://dx.doi.org/10.1023/A:1008192723971
dc.status.ispeerreviewedtrue
dc.subjectBaculovirusen_US
dc.subjectTrichoplusia ni polyhedrin geneen_US
dc.subjectSequence analysisen_US
dc.subjectPhylogenyen_US
dc.titleThe characterization and phylogenetic relationship of the trichoplusia ni single capsid nuclear polyhedrosis virus polyhedrin geneen_US
dc.typeArticleen_US

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