The characterization and phylogenetic relationship of the trichoplusia ni single capsid nuclear polyhedrosis virus polyhedrin gene
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Date
1999
Authors
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Journal ISSN
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Publisher
Kluwer Academic Publishers
Abstract
The polyhedrin gene (polh) was identified from the Trichoplusia ni (Tni) single capsid nuclear polyhedrosis virus (SNPV). An EcoRI fragment containing the truncated polyhedrin gene was detected by hybridization with an AcMNPV expression vector probe; the remaining portion of the gene was amplified by reverse PCR. An open reading frame (ORF) of 741 nucleotides (nt), encoding a putative protein of 246 amino acids (a.a) with Mr 28,780 Da was identified. The 50-noncoding region contained the putative late (TAAG) transcription initiation motif. The 30 end, downstream of the translation stop codon, lacked an obvious putative poly (A) signal. Nucleotide and amino acid homology are greater than 80% to that of Mamestra brassicae polyhedrin sequences.Results suggest that T. niSNPV is a member of the group II nuclear polyhedrosis viruses.
Description
Keywords
Baculovirus, Trichoplusia ni polyhedrin gene, Sequence analysis, Phylogeny
Citation
Fielding, B.C. & Davison, S. (1999). The characterization and phylogenetic relationship of the trichoplusia ni single capsid nuclear polyhedrosis virus polyhedrin gene. Virus Genes, 19(1): 67-72