Use of a rat ex-vivo testis culture method to assess toxicity of select known male reproductive toxicants

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Date

2016

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Elsevier

Abstract

Due to the complex physiology of the testes, in vitro models have been largely unsuccessful at modeling testicular toxicity in vivo. We conducted a pilot study to evaluate the utility of the Durand ex vivo rat seminiferous tubule culture model [1], [2], [3] that supports spermatogenesis through meiosis II, including the formation of round spermatids. We used this system to evaluate the toxicity of four known testicular toxicants: 1,3-dinitrobenzene (DNB), 2-methoxyacetic acid (MAA), bisphenol A (BPA), and lindane over 21 days of culture.

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Keywords

Dinitrobenzene, Methoxyacetic, Testicular toxicity, Bioscience

Citation

Goldstein, K. M. et al. (2016). Use of a rat ex-vivo testis culture method to assess toxicity of select known male reproductive toxicants. Reproductive Toxicology, 60, 92-103. https://doi.org/10.1016/j.reprotox.2016.01.003

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https://doi.org/10.1016/j.reprotox.2016.01.003
 http://hdl.handle.net/10566/8778

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Research Articles (Medical Bioscience)