Using CRISPR/Cas9 to construct consolidated bioprocessing strains from natural isolates of Saccharomyces cerevisiae

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dc.contributor.advisorden Haan, Riaan
dc.contributor.authorMinnaar, Letitia
dc.date.accessioned2023-05-22T09:30:29Z
dc.date.accessioned2024-05-09T07:45:38Z
dc.date.available2023-05-22T09:30:29Z
dc.date.available2024-05-09T07:45:38Z
dc.date.issued2022
dc.description>Magister Scientiae - MScen_US
dc.description.abstractSaccharomyces cerevisiae has gained much attention as a host for cellulosic bioethanol production using consolidated bioprocessing (CBP) methodologies, due to its high ethanol producing titres, heterologous protein producing capabilities, and tolerance to various industry-relevant stresses. Since the secretion profiles of heterologous proteins are relatively low in industrial and laboratory strains of S. cerevisiae, natural isolates may offer a more diverse genetic background with increased robustness to allow for improved heterologous protein secretion. In this study, the potential of natural and industrial S. cerevisiae strains to secrete a core cellulase enzyme complex (CBHI, CBHII, EG and BGL), encoded by genes integrated using CRISPR/Cas9 tools, was evaluated.en_US
dc.identifier.urihttps://hdl.handle.net/10566/13350
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectBiotechnologyen_US
dc.subjectProteinen_US
dc.subjectBioethanolen_US
dc.subjectFood securityen_US
dc.subjectFuel securityen_US
dc.titleUsing CRISPR/Cas9 to construct consolidated bioprocessing strains from natural isolates of Saccharomyces cerevisiaeen_US

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