Using CRISPR/Cas9 to construct consolidated bioprocessing strains from natural isolates of Saccharomyces cerevisiae

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Date

2022

Journal Title

Journal ISSN

Volume Title

Publisher

University of the Western Cape

Abstract

Saccharomyces cerevisiae has gained much attention as a host for cellulosic bioethanol production using consolidated bioprocessing (CBP) methodologies, due to its high ethanol producing titres, heterologous protein producing capabilities, and tolerance to various industry-relevant stresses. Since the secretion profiles of heterologous proteins are relatively low in industrial and laboratory strains of S. cerevisiae, natural isolates may offer a more diverse genetic background with increased robustness to allow for improved heterologous protein secretion. In this study, the potential of natural and industrial S. cerevisiae strains to secrete a core cellulase enzyme complex (CBHI, CBHII, EG and BGL), encoded by genes integrated using CRISPR/Cas9 tools, was evaluated.

Description

>Magister Scientiae - MSc

Keywords

Biotechnology, Protein, Bioethanol, Food security, Fuel security

Citation