Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

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dc.contributor.authorNgara, Rudo
dc.contributor.authorNdimba, Roya
dc.contributor.authorBorch-Jensen, Jonas
dc.contributor.authorJensen, Ole Nørregaard
dc.contributor.authorNdimba, Bongani K.
dc.date.accessioned2013-02-19T11:28:34Z
dc.date.available2013-02-19T11:28:34Z
dc.date.issued2012
dc.description.abstractSorghum bicolor, a drought tolerant cereal crop, is not only an important food source in the semi arid/arid regions but also a potential model for studying and gaining a better understanding of the molecular mechanisms of drought and salt stress tolerance in cereals. In this study, seeds of a sweet sorghumvariety, MN1618, were planted and grown on solid MS growth medium with or without 100mM NaCl. Heat shock protein expression immunoblotting assays demonstrated that this salt treatment induced stress within natural physiological parameters for our experimental material. 2D PAGE in combination with MS/MS proteomics techniques were used to separate, visualise and identify salinity stress responsive proteins in young sorghum leaves. Out of 281 Coomassie stainable spots, 118 showed statistically significant responses (p<0.05) to salt stress treatments. Of the 118 spots, 79 were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and of these, 55 were positively identified. Identified proteins were divided into six functional categories including both known and novel/putative stress responsive proteins. Molecular and physiological functions of some of our proteins of interest are currently under investigation via bioinformatic and molecular biology approaches.en_US
dc.description.accreditationWeb of Scienceen_US
dc.identifier.citationNgara, et al. (2012). Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings. Journal of Proteomics, 75: 4139-4150en_US
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10566/543
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherElsevieren_US
dc.rightsThis is the author postprint version of an article published by Elsevier. Readers may make use of the material providing due attribution is given.
dc.source.urihttp://dx.doi.org/10.1016/j.jprot.2012.05.038
dc.status.ispeerreviewedtrue
dc.subjectSweet sorghumen_US
dc.subjectMN1618en_US
dc.subjectSalinity stressen_US
dc.subjectMALDI-TOF/TOFen_US
dc.subjectMass spectrometryen_US
dc.subjectBiofuelsen_US
dc.titleIdentification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlingsen_US
dc.typeArticleen_US

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