Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings
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Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Sorghum bicolor, a drought tolerant cereal crop, is not only an important food source in the
semi arid/arid regions but also a potential model for studying and gaining a better
understanding of the molecular mechanisms of drought and salt stress tolerance in cereals.
In this study, seeds of a sweet sorghumvariety, MN1618, were planted and grown on solid MS
growth medium with or without 100mM NaCl. Heat shock protein expression immunoblotting
assays demonstrated that this salt treatment induced stress within natural physiological
parameters for our experimental material. 2D PAGE in combination with MS/MS proteomics
techniques were used to separate, visualise and identify salinity stress responsive proteins in
young sorghum leaves. Out of 281 Coomassie stainable spots, 118 showed statistically
significant responses (p<0.05) to salt stress treatments. Of the 118 spots, 79 were selected for
tandem mass spectrometric identification, owing to their good resolution and abundance
levels, and of these, 55 were positively identified. Identified proteins were divided into six
functional categories including both known and novel/putative stress responsive proteins.
Molecular and physiological functions of some of our proteins of interest are currently under
investigation via bioinformatic and molecular biology approaches.
Description
Keywords
Sweet sorghum, MN1618, Salinity stress, MALDI-TOF/TOF, Mass spectrometry, Biofuels
Citation
Ngara, et al. (2012). Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings. Journal of Proteomics, 75: 4139-4150