TUNEL assay—Standardized method for testing sperm DNA fragmentation

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dc.contributor.authorHenkel, R
dc.contributor.authorSharma, R
dc.contributor.authorIovine, C
dc.date.accessioned2021-05-11T11:47:02Z
dc.date.available2021-05-11T11:47:02Z
dc.date.issued2021
dc.description.abstractSperm DNA integrity is important for normal functions such as fertilization, implantation, pregnancy and fetal development. Sperm DNA fragmentation (SDF) is more common in infertile men and may be responsible for poor reproductive function. Although there are a number of tests available to measure SDF, the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling TUNEL) assay using flow cytometry is becoming more popular to measure the sperm DNA fragmentation. It is a direct test that measures both single- and double- DNA strand breaks. In this review, we describe the protocol, quality control and measurement of sperm DNA fragmentation using a benchtop flow cytometer.en_US
dc.identifier.citationHenkel, R. et al. (2021). TUNEL assay—Standardized method for testing sperm DNA fragmentation. Andrologia, 53(2),e13738en_US
dc.identifier.issn0303-4569
dc.identifier.uri10.1111/and.13738
dc.identifier.urihttp://hdl.handle.net/10566/6137
dc.language.isoenen_US
dc.publisherBlackwell Publishing Ltden_US
dc.subjectDNA fragmentationen_US
dc.subjectFlow cytometeren_US
dc.subjectMale infertilityen_US
dc.subjectSpermen_US
dc.titleTUNEL assay—Standardized method for testing sperm DNA fragmentationen_US
dc.typeArticleen_US

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