TUNEL assay—Standardized method for testing sperm DNA fragmentation
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Blackwell Publishing Ltd
Abstract
Sperm DNA integrity is important for normal functions such as fertilization, implantation, pregnancy and fetal development. Sperm DNA fragmentation (SDF) is more common in infertile men and may be responsible for poor reproductive function. Although there are a number of tests available to measure SDF, the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling TUNEL) assay using flow cytometry is becoming more popular to measure the sperm DNA fragmentation. It is a direct test that measures both single- and double- DNA strand breaks. In this review, we describe the protocol, quality control and measurement of sperm DNA fragmentation using a benchtop flow cytometer.
Description
Keywords
DNA fragmentation, Flow cytometer, Male infertility, Sperm
Citation
Henkel, R. et al. (2021). TUNEL assay—Standardized method for testing sperm DNA fragmentation. Andrologia, 53(2),e13738