Efficient biosynthesis of 3-hydroxypropionic acid from glucose through multidimensional engineering of escherichia coli

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dc.contributor.authorZhang, Yufei
dc.contributor.authorYun, Junhua
dc.contributor.authorParvez, Amreesh
dc.date.accessioned2025-02-11T07:42:37Z
dc.date.available2025-02-11T07:42:37Z
dc.date.issued2023
dc.description.abstract3-Hydroxypropionic acid (3-HP) is a top value-added chemical with multifaceted application in chemical, material, and food field. However, limited availability of robust strains and elevated fermentation costs currently impose constraints on sustainable biosynthesis of 3-HP. Herein, transporter engineering, metabolic dynamic modulation, and enzyme engineering were combined to address above limitations. First, a glucose-utilizing 3-HP biosynthetic pathway was constructed in Escherichia coli, followed by recruiting alternative glucose transport system to overcome center metabolism overflow. Next, the Cra (a transcription factor)-dependent switch was applied to autonomously fine-tune carbon flux, which alleviated growth retardation and improved the 3-HP production. Subsequently, inactivation of glycerol facilitator (GlpF) increased intracellular glycerol levels and boosted 3-HP biosynthesis, but caused toxic intermediate 3-hydroxypropionaldehyde (3-HPA) accumulation. Furthermore, semi-rational design of aldehyde dehydrogenase (YdcW) increased its activity and eliminated 3-HPA accumulation. Finally, fed-batch fermentation of the final strain resulted in 52.73 g/L 3-HP, with a yield of 0.59 mol/mol glucose.
dc.identifier.citationZhang, Y., Yun, J., Zhang, G., Parvez, A., Zhou, L., Zabed, H.M., Li, J. and Qi, X., 2023. Efficient biosynthesis of 3-hydroxypropionic acid from glucose through multidimensional engineering of Escherichia coli. Bioresource Technology, 389, p.129822.
dc.identifier.urihttps://doi.org/10.1016/j.biortech.2023.129822
dc.identifier.urihttps://hdl.handle.net/10566/19986
dc.language.isoen
dc.publisherElsevier Ltd
dc.subject3-Hydroxypropionic acid
dc.subjectBiosynthesis
dc.subjectEnzyme semi-rational design
dc.subjectGlucose valorization
dc.subjectMultidimensional engineering
dc.titleEfficient biosynthesis of 3-hydroxypropionic acid from glucose through multidimensional engineering of escherichia coli
dc.typeArticle

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