Research Articles (Biotechnology)

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    Farmers’ Knowledge, Perceptions and Attitudes on Crop-Dairy Goat Integration Farming System in Elgeyo Marakwet County
    (Multidisciplinary Digital Publishing Institute (MDPI), 2024) Keyster, Marshall; Cheboi, Juliana; Greathead, Henry
    Several interventions have been promoted in dry areas to improve food and nutrition insecurity. However, studies on the key drivers influencing adoption and uptake are limited. Therefore, research was undertaken to investigate farmers’ knowledge, perceptions and attitudes on an integrated crop–dairy goat farming system in Elgeyo Marakwet. A cross-sectional study entailing a household survey of 201 respondents, six key informant interviews and eight focus group discussions was undertaken. This study utilized a multi-stage sampling procedure to sample the farmers and calculated the sample size using Krejcie and Morgan tables. Quantitative data were subjected to descriptive and inferential statistics using SPSS software version 22, while qualitative data were analysed using N-vivo software version 10 through the Framework Analysis method. The findings show that drought (84.6%), change in rainfall pattern (77.6%), farm size (57.2%), unavailability of quality seeds (52.2%), fodder acreage (58.7%), diseases (69.7%) and pest severity (68.7%) are the principal drivers for adoption of the integrated crop–dairy goat farming system. Dairy goats are associated with women in this community since they are regarded as small animals and have no monetary value, hence increasing the participation of women in the access, control and decision making of agricultural resources. To increase adoption, strategies focusing on improving water supply, quality seeds, agro-veterinary services and production are advocated.
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    Ethical considerations for Forensic Genetic Frequency databases: First Report conception and development
    (Elsivier, 2024) D’Amato, Maria E; Joly, Y; Lynch, V
    The Forensic Databases Advisory Board (FDAB), an independent board that assists the International Society for Forensic Genetics (ISFG), has presented a First Report on ethical aspects of the following Forensic Genetic Frequency Databases (FGFD): EMPOP, STRidER and YHRD. The FDAB designed an ethical framework to evaluate the content of these FGFD, and the factors to be considered for retention and acceptance of submissions. The FDAB framework proposes to categorize submissions according to the risk of having contravened the universal ethical principles outlined by international organizations, and the guidelines adopted by the ISFG. The report has been open to discussion by the scientific community since 2023. Herein we present the conception and development of the First Report along with a summary of its content, with consideration of the feedback received.
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    Molecular modeling and simulation studies of selex-derived high-affinity dna aptamers to the ebola virus nucleoprotein
    (Taylor and Francis Ltd., 2024) Martin, Darius Riziki; Madiehe, Abram; Meyer, Mervin
    Ebola viral disease (EVD) is a highly infectious and potentially fatal illness with a case fatality rate ranging from 25% to 90%. To effectively control its spread, there is a need for rapid, reliable and lowcost point-of-care (P OC) diagnostic tests. While various EVD diagnostic tests exist, few are P OC tests, and many are not cost-effective. The use of antibodies in these tests has limitations, prompting the exploration of aptamers as potential alternatives. Various proteins from the Ebola virus (EBOV) proteome, including EBOV nucleoprotein (NP), are considered viable targets for diagnostic assays. A previous study identified three aptamers (Apt1. Apt2 and Apt3) with high affinity for EBOV NP using systemic evolution of ligands by exponential enrichment (SELEX). This study aimed to employ in silico methods, such as Phyre2, RNAfold, RNAComposer, HADDOCK and GROMACS, to model the structures of EBOV NP and the aptamers, and to investigate their binding. The in silico analysis revealed successful binding of all the three aptamers to EBOV NP, with a suggested ranking of Apt1 > Apt2 > Apt3 based on binding affinity. Microscale thermophoresis (MST) analysis confirmed the binding, providing dissociation constants of 25 ± 2.84, 56 ± 2.76 and 140 ±3.69 nM for Apt1, Apt2 and Apt3, respectively. The study shows that the findings of the in silico analysis was in agreement with the MST analysis. Inclusion of these in silico approaches in diagnostic assay development can expedite the selection of candidate aptamers, potentially overcoming challenges associated with aptamer application in diagnostics.
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    Heavy metal stress and mitogen activated kinase transcription factors in plants: Exploring heavy metal-ros influences on plant signalling pathways
    (John Wiley and Sons Inc, 2024) Niekerk, Lee-Ann; Basson, Gerhard; Klein, Ashwil; Keyster, Marshall
    Due to their stationary nature, plants are exposed to a diverse range of biotic and abiotic stresses, of which heavy metal (HM) stress poses one of the most detrimental abiotic stresses, targeting diverse plant processes. HMs instigate the overproduction of reactive oxygen species (ROS), and to mitigate the adverse effects of ROS, plants induce multiple defence mechanisms. Besides the negative implications of overproduction of ROS, these molecules play a multitude of signalling roles in plants, acting as a central player in the complex signalling network of cells. One of the ROS-associated signalling mechanisms is the mitogen-activated protein kinase (MAPK) cascade, a signalling pathway which transduces extracellular stimuli into intracellular responses. Plant MAPKs have been implicated in signalling involved in stress response, phytohormone regulation, and cell cycle cues. However, the influence of various HMs on MAPK activation has not been well documented. In this review, we address and summarise several aspects related to various HM-induced ROS signalling. Additionally, we touch on how these signals activate the MAPK cascade and the downstream transcription factors that influence plant responses to HMs. Moreover, we propose a workflow that could characterise genes associated with MAPKs and their roles during plant HM stress responses.