Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation
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Date
2023
Authors
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Publisher
Environmental Pollution
Abstract
Petroleum hydrocarbons and heavy metals are some of the most widespread contaminants affecting marine
ecosystems, urgently needing effective and sustainable remediation solutions. Microbial-based bioremediation is
gaining increasing interest as an effective, economically and environmentally sustainable strategy. Here, we
hypothesized that the heavily polluted coastal area facing the Sarno River mouth, which discharges >3 tons of
polycyclic aromatic hydrocarbons (PAHs) and ~15 tons of heavy metals (HMs) into the sea annually, hosts
unique microbiomes including marine bacteria useful for PAHs and HMs bioremediation. We thus enriched the
microbiome of marine sediments, contextually selecting for HM-resistant bacteria. The enriched mixed bacterial
culture was subjected to whole-DNA sequencing, metagenome-assembled-genomes (MAGs) annotation, and
further sub-culturing to obtain the major bacterial species as pure strains.
Description
Keywords
polycyclic aromatic hydrocarbons, heavy metals, bioremediation, marine biotechnology, next-generation sequencing
Citation
Dell’Anno, F., van Zyl, L.J., Trindade, M., Buschi, E., Cannavacciuolo, A., Pepi, M., Sansone, C., Brunet, C., Ianora, A., de Pascale, D. and Golyshin, P.N., 2023. Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation. Environmental Pollution, 317, p.120772.