Carcinoembryonic antigen immunosensor developed with organoclay nanogold composite film
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
ESG
Abstract
Organoclay nanogold composite were prepared using gold nanoparticles and the natural Cameroonian clay grafted with amino organosilane. The functionnalization of clay provided abundant amino group to assemble gold nanoparticles. A label-free electrochemical immunosensor for the sensitive determination of carcinoembryonic antigen (CEA) was fabricated by immobilizing anti-CEA onto organoclay nanogold composite film modified electrode by the cross-linking method using glutaraldehyde. In addition, the preparation procedure of the immunosensor was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, the resulting immunosensor displayed a high sensitivity for the detection of CEA, and responded to the CEA concentration in two ranges from 0.05 to 5.0 ng/mL (R = 0.991) and from 5.0 to 120.0 ng/mL (R = 0.998) with a detection limit of 0.01 ng/mL.
Description
Keywords
Organoclay, Nanogold composite, Modified electrode, Immunosenor, Carcinoembryonic antigen
Citation
Kemmegne-Mbouguen, J.C. et al. (2014). Carcinoembryonic antigen immunosensor developed with organoclay nanogold composite film. International Journal of Electrochemical Science, 9: 478 - 492