Afriplex GRTTM extract attenuates hepatic steatosis in an in vitro model of NAFLD

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dc.contributor.authorGabuza, Kwazi
dc.contributor.authorMabuda, Thendo Innocent
dc.contributor.authorPatel, Oelfah
dc.date.accessioned2024-11-20T08:26:44Z
dc.date.available2024-11-20T08:26:44Z
dc.date.issued2024
dc.description.abstractCurrently, it is acknowledged that vitamin E, insulin sensitizers and anti-diabetic drugs are used to manage non-alcoholic fatty liver disease (NAFLD), however, these therapeutic interventions harbour adverse side effects. Pioglitazone, an anti-diabetic drug, is currently the most effective therapy to manage NAFLD. The use of natural medicines is widely embraced due to the lack of evidence of their negative side effects. Rooibos has been previously shown to decrease inflammation and oxidative stress in experimental models of diabetes, however, this is yet to be explored in a setting of NAFLD. This study was aimed at investigating the effects of an aspalathin-rich green rooibos extract (Afriplex GRTTM) against markers of hepatic oxidative stress, inflammation and apoptosis in an in vitro model of NAFLD. Oleic acid [1 mM] was used to induce hepatic steatosis in C3A liver cells. Thereafter, the therapeutic effect of Afriplex GRTTM, with or without pioglitazone, was determined by assessing its impact on cell viability, changes in mitochondrial membrane potential, intracellular lipid accumulation and the expression of genes and proteins (ChREBP, SREBF1, FASN, IRS1, SOD2, Caspase-3, GSTZ1, IRS1 and TNF-α) that are associated with the development of NAFLD.
dc.identifier.citationGabuza, K., Mabuda, T.I., Patel, O., Khuboni, N., van Aarde, R., Riedel, S., Sangweni, N.F., Windvogel, S., Johnson, R. and Muller, C.J., 2024. Afriplex GRTTM extract attenuates hepatic steatosis in an in vitro model of NAFLD. Plos one, 19(4), p.e0297572.
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0297572
dc.identifier.urihttps://hdl.handle.net/10566/19702
dc.language.isoen
dc.publisherPublic Library of Science
dc.subjectCaspase 3
dc.subjectalpha tocopherol
dc.subjectapoptosis
dc.subjectfatty acid synthase
dc.subjectSTZ1 protein
dc.titleAfriplex GRTTM extract attenuates hepatic steatosis in an in vitro model of NAFLD
dc.typeArticle

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