Molecular detection and genetic manipulation of the Black Queen Cell Virus

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dc.contributor.advisorDavison, Sean
dc.contributor.authorBenjeddou, Mongi
dc.contributor.otherDept. of Biotechnology
dc.contributor.otherFaculty of Science
dc.date.accessioned2013-05-28T08:48:35Z
dc.date.accessioned2024-05-09T08:19:12Z
dc.date.available2007/03/16 15:48
dc.date.available2007/03/16
dc.date.available2013-05-28T08:48:35Z
dc.date.available2024-05-09T08:19:12Z
dc.date.issued2002
dc.descriptionPhilosophiae Doctor - PhDen_US
dc.description.abstractThe South African isolate of the Black Queen-Cell Virus (BQCV), a honeybee virus, was previously found to have an 8550 nucleotide genome excluding the poly(A) tail. Its genome contained two ORFs, a 5'-proximal ORF encoding a putative replicase protein and a 3'-proximal ORF encoding a capsid polyprotein.A reverse transcriptase PCR (RT -PCR) assay was developed for the detection of BQCV and acute bee-paralysis virus (ABPV). Complete genomes sequences w ere used to design unique PCR primers within a l-kb region from the 3' end of both genomes to amplify a fragment of 70.0 bp from BQCV and 900 bp from ABPV. The combined guanidinium thiocyanate and silica membrane method was used to extract total RNA from samples of healthy and laboratory-infected bee pupae. In a blind test, RT-PCR successfully identified the samples containing BQCV and ABPV. Sensitivities were of the order of 130 genome equivalents of purified BQCV and 1600 genome equivalents of ABPV.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/13496
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectVirologyen_US
dc.subjectVirusesen_US
dc.subjectHoneybeeen_US
dc.subjectDiseasesen_US
dc.subjectBeesen_US
dc.titleMolecular detection and genetic manipulation of the Black Queen Cell Virusen_US
dc.typeThesisen_US

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