HPLC determination of selected flavonoid glycosides and their corresponding aglycones in Sutherlandia frutescens materials

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dc.contributor.authorMbamalu, Oluchi
dc.contributor.authorAntunes, E.
dc.contributor.authorSilosini, N.
dc.contributor.authorSamsodien, Halima
dc.contributor.authorSyce, James
dc.date.accessioned2016-05-12T13:15:41Z
dc.date.available2016-05-12T13:15:41Z
dc.date.issued2016
dc.description.abstractSutherlandia frutescens is a popular South African plant commercially available in a range of formulations. However, reference standards for quality and stability assessment are lacking. This work reports the development and validation of a reversed phase HPLC method for the analysis of flavonoid glycosides and their corresponding aglycones in S. frutescens products. Five materials containing either leaf powder (LP) or spray-dried aqueous extract (SDAE) of S. frutescens were analysed for flavonoid content. A primary objective was to isolate non-commercially available flavonoid glycoside compounds (sutherlandins) for use as reference standards. Sutherlandins A, B, C and D were successfully isolated, and used, with other flavonoid compounds for HPLC assay development. The developed HPLC method was linear in the range of 0.2 to 60 µg/ml for quercitrin; 0.2 to 120 µg/ml for quercetin and kaempferol; 0.2 to 200 µg/ml for rutin and kaempferol-3-O-rutinoside; 4 to 180 µg/ml for sutherlandins A and D; and 4 to 200 µg/ml for sutherlandins B and C. Percentage content of sutherlandins A, B, C and D, quercetin and kaempferol in different plant materials were significantly different (P<0.001). The developed HPLC method is simple, precise and robust; and can be employed for the simultaneous determination of flavonoid glycosides and aglycones for quality control of S. frutescens products.en_US
dc.identifier.citationMbamalu, O.N. et al. (2016). HPLC determination of selected flavonoid glycosides and their corresponding aglycones in Sutherlandia frutescens materials. Medicinal & Aromatic Plants, 5(3): 1-10
dc.identifier.issn2167-0412
dc.identifier.urihttp://hdl.handle.net/10566/2183
dc.identifier.urihttp://dx.doi.org/10.4172/2167-0412.1000246
dc.language.isoenen_US
dc.privacy.showsubmitterFALSE
dc.publisherOMICSen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttp://dx.doi.org/10.4172/2167-0412.1000246
dc.status.ispeerreviewedTRUE
dc.subjectSutherlandia frutescensen_US
dc.subjectLegumeen_US
dc.subjectFlavonoiden_US
dc.subjectHerbal medicineen_US
dc.subjectQuality controlen_US
dc.subjectValidationen_US
dc.subjectHigh-performance liquid chromatography (HPLC)
dc.titleHPLC determination of selected flavonoid glycosides and their corresponding aglycones in Sutherlandia frutescens materials
dc.typeArticle

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