HPLC determination of selected flavonoid glycosides and their corresponding aglycones in Sutherlandia frutescens materials
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Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
OMICS
Abstract
Sutherlandia frutescens is a popular South African plant commercially available in a range of formulations.
However, reference standards for quality and stability assessment are lacking. This work reports the development
and validation of a reversed phase HPLC method for the analysis of flavonoid glycosides and their corresponding
aglycones in S. frutescens products. Five materials containing either leaf powder (LP) or spray-dried aqueous extract
(SDAE) of S. frutescens were analysed for flavonoid content. A primary objective was to isolate non-commercially
available flavonoid glycoside compounds (sutherlandins) for use as reference standards. Sutherlandins A, B, C and D
were successfully isolated, and used, with other flavonoid compounds for HPLC assay development. The developed
HPLC method was linear in the range of 0.2 to 60 µg/ml for quercitrin; 0.2 to 120 µg/ml for quercetin and kaempferol;
0.2 to 200 µg/ml for rutin and kaempferol-3-O-rutinoside; 4 to 180 µg/ml for sutherlandins A and D; and 4 to 200 µg/ml
for sutherlandins B and C. Percentage content of sutherlandins A, B, C and D, quercetin and kaempferol in different
plant materials were significantly different (P<0.001). The developed HPLC method is simple, precise and robust; and
can be employed for the simultaneous determination of flavonoid glycosides and aglycones for quality control of S.
frutescens products.
Description
Keywords
Sutherlandia frutescens, Legume, Flavonoid, Herbal medicine, Quality control, Validation, High-performance liquid chromatography (HPLC)
Citation
Mbamalu, O.N. et al. (2016). HPLC determination of selected flavonoid glycosides and their
corresponding aglycones in Sutherlandia frutescens materials. Medicinal & Aromatic Plants, 5(3): 1-10