Nutritional and physiological demands shape the gut microbiome of female world tour cyclists

dc.contributor.authorMeeusen, Romain
dc.contributor.authorAmpe, Toon
dc.contributor.authorDecroix, Lieselot
dc.date.accessioned2025-12-11T12:59:18Z
dc.date.available2025-12-11T12:59:18Z
dc.date.issued2025
dc.description.abstracthis cross-sectional study investigated whether elite female World Tour cyclists have a specific gut microbiome compared to non-athlete female controls, potentially resulting from the unique physiological and dietary demands of high-level endurance cycling. Fourteen female cyclists and thirteen matched controls provided fecal samples during a period of reduced training (off-season cycling). The samples were analyzed using 16S rRNA gene sequencing and short-chain fatty acid (SCFA) quantification. The results revealed significant differences in microbiome composition. The cyclists showed a higher abundance of Bacteroidota (72.7% vs. 15.3%) and a lower abundance of Firmicutes (22.1% vs. 62.5%) compared to the controls, along with reduced alpha-diversity (Shannon index, p < 0.05). Fiber-fermenting families such as Lachnospiraceae and Ruminococcaceae were depleted, consistent with a carbohydrate-focused and relatively low-fiber diet. Interestingly, fecal SCFA levels did not differ, suggesting functional adaptation of the microbiome. These findings indicate that the elite female cyclists may have developed a “performance-adapted” gut microbiome. However, due to the cross-sectional design, causality cannot be established, and the long-term health implications remain uncertain.
dc.identifier.citationAmpe, T., Decroix, L., De Pauw, K., Meeusen, R., Demuyser, T. and Roelands, B., 2025. Nutritional and Physiological Demands Shape the Gut Microbiome of Female World Tour Cyclists. Microorganisms, 13(10), p.2345.
dc.identifier.urihttps://doi.org/10.3390/microorganisms13102345
dc.identifier.urihttps://hdl.handle.net/10566/21575
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.subjectcycling performance
dc.subjectfemale cyclists
dc.subjectgut health
dc.subjectgut microbiome
dc.subjectnutrition
dc.titleNutritional and physiological demands shape the gut microbiome of female world tour cyclists
dc.typeArticle

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