Quantum dot nanotoxicity investigations using human lung cells and toxor electrochemical enzyme assay methodology

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Date

2017

Journal Title

Journal ISSN

Volume Title

Publisher

American Chemical Society

Abstract

Recent studies have suggested that certain nanomaterials can interfere with optically based cytotoxicity assays resulting in underestimations of nanomaterial toxicity. As a result there has been growing interest in the use of whole cell electrochemical biosensors for nanotoxicity applications. Herein we report application of an electrochemical cytotoxicity assay developed in house (TOXOR) in the evaluation of toxic effects of mercaptosuccinic acid capped cadmium telluride quantum dots (MSA capped CdTe QDs), toward mammalian cells. MSA capped CdTe QDs were synthesized, characterized, and their cytotoxicity toward A549 human lung epithelial cells investigated. The internalization of QDs within cells was scrutinized via confocal microscopy.

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Keywords

Chemistry, Electrochemical, Biosensors, Nanotoxicity, Pharmaceuticals

Citation

O’Hara, T. et al. (2017). Quantum dot nanotoxicity investigations using human lung cells and toxor electrochemical enzyme assay methodology. ACS Sensors, 2 (1) ,165-171. https://doi.org/10.1021/acssensors.6b00673