Stress modulation as a means to improve yeasts for lignocellulose bioconversion
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
The second-generation (2G) fermentation environment for lignocellulose conversion presents unique challenges to the fermentative organism that do not necessarily exist in other industrial fermentations. While extreme osmotic, heat, and nutrient starvation stresses are observed in sugar- and starch-based fermentation environments, additional pre-treatment-derived inhibitor stress,
potentially exacerbated by stresses such as pH and product tolerance, exist in the 2G environment. Furthermore, in a consolidated
bioprocessing (CBP) context, the organism is also challenged to secrete enzymes that may themselves lead to unfolded protein
response and other stresses. This review will discuss responses of the yeast Saccharomyces cerevisiae to 2G-specific stresses and
stress modulation strategies that can be followed to improve yeasts for this application. We also explore published –omics data
and discuss relevant rational engineering, reverse engineering, and adaptation strategies, with the view of identifying genes or
alleles that will make positive contributions to the overall robustness of 2G industrial strains.
Description
Keywords
Stress, Yeasts, Second-generation biofuel, Nutrient starvation
Citation
Brandt, B. et al. (2021). Stress modulation as a means to improve yeasts for lignocellulose bioconversion. Applied Microbiology and Biotechnology, 105. 4899-4918. https://doi.org/10.1007/s00253-021-11383-y