Characterization and expression analysis of heme oxygenase genes from sorghum bicolor

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dc.contributor.authorMulaudzi-Masuku, Takalani
dc.contributor.authorIkebudu, Vivian
dc.contributor.authorMuthevhuli, Mpho
dc.date.accessioned2023-06-02T09:36:55Z
dc.date.available2023-06-02T09:36:55Z
dc.date.issued2019
dc.description.abstractHeme oxygenases (HOs) have a major role in phytochrome chromophore biosynthesis, and chromophores in turn have antioxidant properties. Plant heme oxygenases are divided into the HO1 sub-family comprising HO1, HO3, and HO4, and the HO2 sub-family, which consists of 1 member, HO2. This study identified and characterized 4 heme oxygenase members from Sorghum bicolor. Multiple sequence alignments showed that the heme oxygenase signature motif (QAFICHFYNI/V) is conserved across all SbHO proteins and that they share above 90% sequence identity with other cereals. Quantitative real-time polymerase chain reaction revealed that SbHO genes were expressed in leaves, stems, and roots, but most importantly their transcript level was induced by osmotic stress, indicating that they might play a role in stress responses. These findings will strengthen our understanding of the role of heme oxygenases in plant stress responses and may contribute to the development of stress tolerant crops.en_US
dc.identifier.citationMulaudzi-Masuku, T. et al. (2019). Characterization and expression analysis of heme oxygenase genes from sorghum bicolor. Bioinformatics and Biology Insights, 13. 10.1177/1177932219860813en_US
dc.identifier.issn1177-9322
dc.identifier.uri10.1177/1177932219860813
dc.identifier.urihttp://hdl.handle.net/10566/8993
dc.language.isoenen_US
dc.publisherSAGE Publicationsen_US
dc.subjectBiotechnologyen_US
dc.subjectSorghum bicoloren_US
dc.subjectHeme oxygenaseen_US
dc.subjectOsmotic stressen_US
dc.subjectBioinformaticsen_US
dc.titleCharacterization and expression analysis of heme oxygenase genes from sorghum bicoloren_US
dc.typeArticleen_US

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