Characterization and expression analysis of heme oxygenase genes from sorghum bicolor
Loading...
Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
SAGE Publications
Abstract
Heme oxygenases (HOs) have a major role in phytochrome chromophore biosynthesis, and chromophores in turn have antioxidant
properties. Plant heme oxygenases are divided into the HO1 sub-family comprising HO1, HO3, and HO4, and the HO2 sub-family, which
consists of 1 member, HO2. This study identified and characterized 4 heme oxygenase members from Sorghum bicolor. Multiple sequence
alignments showed that the heme oxygenase signature motif (QAFICHFYNI/V) is conserved across all SbHO proteins and that they share above
90% sequence identity with other cereals. Quantitative real-time polymerase chain reaction revealed that SbHO genes were expressed in
leaves, stems, and roots, but most importantly their transcript level was induced by osmotic stress, indicating that they might play a role in stress
responses. These findings will strengthen our understanding of the role of heme oxygenases in plant stress responses and may contribute to
the development of stress tolerant crops.
Description
Keywords
Biotechnology, Sorghum bicolor, Heme oxygenase, Osmotic stress, Bioinformatics
Citation
Mulaudzi-Masuku, T. et al. (2019). Characterization and expression analysis of heme oxygenase genes from sorghum bicolor. Bioinformatics and Biology Insights, 13. 10.1177/1177932219860813