Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing

Simple item page
dc.contributor.authorKelch, Jessica
dc.contributor.authorDelaney, Aoife
dc.contributor.authorBaker, Priscilla
dc.date.accessioned2022-06-13T09:30:46Z
dc.date.available2022-06-13T09:30:46Z
dc.date.issued2020
dc.description.abstractPreparation and electrochemical interrogation of a novel redox active progesterone derivative progesterone thiosemicarbazone (PATC) is presented here together with an investigation into its suitability as conjugate in progesterone hormone immunosensing. PATC synthesis involved a condensation reaction between progesterone acetate and thiosemicarbazone hydrochloride. Voltammetric and pulse techniques confirmed the redox behav- iour of the new compound with concentration and scan rate dependant irreversible behaviour evident at glassy carbon and gold transducers - ko (standard heterogeneous rate constant) was 2.56 � 10-3 cm2/s (ν ¼ 100 mV/s in non-aqeuous media). Bioaffinity studies towards anti-progesterone antibodies involved a competitive ELISA format (optical) which confirmed recognition of the new progesterone derivative. Electrochemical impedance spectroscopy was employed as an interrogation technique in order to establish optimum binding and surface conditions for progesterone antigen-antibody interaction with the assistance of a redox probe (potassium hexacyanoferrate).en_US
dc.identifier.citationKelch, J. et al. (2020). Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing. Biosensors and Bioelectronics, 150, 111876. https://doi.org/10.1016/j.bios.2019.111876en_US
dc.identifier.issn0956-5663
dc.identifier.urihttps://doi.org/10.1016/j.bios.2019.111876
dc.identifier.urihttp://hdl.handle.net/10566/7511
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectElectrochemicalen_US
dc.subjectImmunosensingen_US
dc.subjectProgesteroneen_US
dc.subjectHormoneen_US
dc.titleImpedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensingen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
kelch_impedimetric and electrochemical evaluation_2020.pdf
Size:
1.82 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles (Chemistry)