Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Preparation and electrochemical interrogation of a novel redox active progesterone derivative progesterone
thiosemicarbazone (PATC) is presented here together with an investigation into its suitability as conjugate in
progesterone hormone immunosensing. PATC synthesis involved a condensation reaction between progesterone
acetate and thiosemicarbazone hydrochloride. Voltammetric and pulse techniques confirmed the redox behav-
iour of the new compound with concentration and scan rate dependant irreversible behaviour evident at glassy
carbon and gold transducers - ko (standard heterogeneous rate constant) was 2.56 � 10-3 cm2/s (ν ¼ 100 mV/s in
non-aqeuous media). Bioaffinity studies towards anti-progesterone antibodies involved a competitive ELISA
format (optical) which confirmed recognition of the new progesterone derivative. Electrochemical impedance
spectroscopy was employed as an interrogation technique in order to establish optimum binding and surface
conditions for progesterone antigen-antibody interaction with the assistance of a redox probe (potassium
hexacyanoferrate).
Description
Keywords
Electrochemical, Immunosensing, Progesterone, Hormone
Citation
Kelch, J. et al. (2020). Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing. Biosensors and Bioelectronics, 150, 111876. https://doi.org/10.1016/j.bios.2019.111876