High-quality genome assembly and annotation of Thalassiosira rotula (synonym of Thalassiosira gravida)

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Date

2025

Journal Title

Journal ISSN

Volume Title

Publisher

Nature Research

Abstract

Diatoms are unicellular eukaryotic microorganisms thriving in most aquatic environments thanks to the expression of biosynthetic pathways for secondary metabolites involved in defence and adaptation to environmental changes. The sequencing of the transcriptome of the cosmopolitan diatom Thalassiosira rotula Meunier 1910 (synonym of Thalassiosira gravida Cleve 1896) and of the metagenome of its associated microbiome revealed the presence of biosynthetic pathways synthesising molecules and compounds useful for the algae survival and with potential biotechnological applications. Here we present the genome of a Neapolitan T. rotula strain, which is 672 Mbp in size due to a high proportion of repetitive elements (63.59%) and segmental duplications (14%), while the number of predicted genes resulted to be comparable to that of smaller diatom genomes. DNA methylation was predominantly located in transposable elements.

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Keywords

Diatoms, DNA Methylation, DNA Transposable Elements, Genome, Metagenome

Citation

Di Costanzo, F., Di Marsico, M., Orefice, I., Kristoffersen, J.B., Kasapidis, P., Chaumier, T., Ambrosino, L., Miralto, M., Aiese Cigliano, R., Verret, F. and Tirichine, L., 2025. High-quality genome assembly and annotation of Thalassiosira rotula (synonym of Thalassiosira gravida). Scientific Data, 12(1), p.310.