Novel 3-mercaptopropionic acid capped iridium selenide quantum dots modified electrochemical immunosensor for the detection of fish toxin, nodularin
| dc.contributor.advisor | Iwuoha, Emmanuel | |
| dc.contributor.advisor | Baker, Priscilla | |
| dc.contributor.author | Nxusani, Ezo | |
| dc.date.accessioned | 2015-11-03T13:30:10Z | |
| dc.date.accessioned | 2024-05-09T10:50:35Z | |
| dc.date.available | 2015-11-03T13:30:10Z | |
| dc.date.available | 2024-05-09T10:50:35Z | |
| dc.date.issued | 2012 | |
| dc.description | >Magister Scientiae - MSc | en_US |
| dc.description.abstract | A novel 3-mercaptopropionic acid capped iridium selenide quantum dots based label free impedimetric immunosensor was successfully constructed. The 3-mercaptopropionic acid capped iridium selenide quantum dots synthesized were studied using HRTEM, revealing the formation of very small sizes, of about 3 nm. The optical Uv-Vis absorption wavelength of the quantum dots is blue-shifted, a phenomenon explained by the effective mass approximation (EMA) for semiconducting materials with sizes below 10 nm. Using cyclic voltammetry it is noted that the quantum dots have interesting electro-catalytical properties. The immunosensor proved to be sensitive towards nodularin, with a very low detection limit of 0.009 ng/mL and is significantly lower than the recent anti-nodularin ELISA kit developed by (Zhou et al., 2011) which has a detection limit of 0.16 ng/mL.Also the dection limit of the immunosensor is below the South African guideline value for microcystin-LR (0-0.8) μg/L (DWAF; 1996). The calibration curve of the 3MPA-GaSe nanocrystal based biosensor was successfully constructed, which exhibited a trend described by Michaelis-Menten, a typical behaviour of enzymatic biosensors. The detection limit of the biosensor is 0.004 nM and is significantly lower than the action limit of 17beta-estradiol, (1.47 x 10-10 M). | en_US |
| dc.identifier.uri | https://hdl.handle.net/10566/14536 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of the Western Cape | en_US |
| dc.rights.holder | University of the Western Cape | en_US |
| dc.subject | Iridium selenide | en_US |
| dc.subject | Quantum dots | en_US |
| dc.subject | Immunosensor | en_US |
| dc.title | Novel 3-mercaptopropionic acid capped iridium selenide quantum dots modified electrochemical immunosensor for the detection of fish toxin, nodularin | en_US |
| dc.type | Thesis | en_US |