Human sperm handling in intracytoplasmic sperm injection processes: In vitro studies on mouse oocyte activation, embryo development competence and sperm oxidation–reduction potential

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dc.contributor.authorRoychoudhury, S.
dc.contributor.authorMaldonado-Rosas, I.
dc.contributor.authorAgarwal, A.
dc.contributor.authorEsteves, S.C.
dc.contributor.authorHenkel, Ralf
dc.contributor.authorSharma, Rajan
dc.date.accessioned2018-01-17T10:33:25Z
dc.date.available2018-01-17T10:33:25Z
dc.date.issued2018
dc.description.abstractPolyvinylpyrrolidone (PVP) and hyaluronic acid (HA) are routinely used in handling spermatozoa for intracytoplasmic sperm injection (ICSI). As there are still concerns about possible adverse effects on the embryo, this study investigated sperm handling in a mouse ICSI model to (i) evaluate oocyte activation after injection of spermatozoa selected for rotational or linear motion in PVP; (ii) assess the effect of sperm selection in PVP, HA and medium on oocyte activation; (iii) examine the effects of PVP and HA on parthenogenetic oocyte activation and embryo development; and (iv) assess the oxidation–reduction potential (ORP) of spermatozoa exposed to PVP, HA or medium. Oocyte activation was higher when spermatozoa exhibited rotational motion rather than linear motion (79% vs. 52%; p = .05). There was no difference in oocyte activation and embryo development after parthenogenetic oocyte activation after sperm injection using PVP, HA or medium-incubated spermatozoa. PVP-selected spermatozoa exhibited lower (p < .0001) ORP levels than using HA. Thus, results indicate that the sperm handling method and the type of medium used impact ICSI outcomes. Overall, sperm incubation in PVP, HA and medium yields similar outcomes with regard to oocyte activation and embryo development. However, PVP provides more antioxidative protection than HA and should therefore be preferred for sperm manipulation.en_US
dc.identifier.citationRoychoudhury, S. et al. (2018). Human sperm handling in intracytoplasmic sperm injection processes: In vitrostudies on mouse oocyte activation, embryo development competence and sperm oxidation–reduction potential. Andrologia, 2018: e12943en_US
dc.identifier.issn0303-4569
dc.identifier.urihttp://dx.doi.org/10.1111/and.12943
dc.identifier.urihttp://hdl.handle.net/10566/3393
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectEmbryo developmenten_US
dc.subjectHuman sperm handlingen_US
dc.subjectIntracytoplasmic sperm injectionen_US
dc.subjectMouse oocyteen_US
dc.subjectOxidation–reduction potentialen_US
dc.titleHuman sperm handling in intracytoplasmic sperm injection processes: In vitro studies on mouse oocyte activation, embryo development competence and sperm oxidation–reduction potentialen_US
dc.typeArticleen_US

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