Structure and biosynthesis of carotenoids produced by a novel Planococcus sp. isolated from South Africa

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dc.contributor.authorMoyo, Anesu Conrad
dc.contributor.authorDufossé, Laurent
dc.contributor.authorGiuffrida, Daniele
dc.date.accessioned2022-09-19T07:14:58Z
dc.date.available2022-09-19T07:14:58Z
dc.date.issued2022
dc.description.abstractThe genus Planococcus is comprised of halophilic bacteria generally reported for the production of carotenoid pigments and biosurfactants. In previous work, we showed that the culturing of the orange-pigmented Planococcus sp. CP5-4 isolate increased the evaporation rate of industrial wastewater brine effluent, which we attributed to the orange pigment. This demonstrated the potential application of this bacterium for industrial brine effluent management in evaporation ponds for inland desalination plants. Here we identified a C30- carotenoid biosynthetic gene cluster responsible for pigment biosynthesis in Planococcus sp. CP5-4 through isolation of mutants and genome sequencing. We further compare the core genes of the carotenoid biosynthetic gene clusters identified from different Planococcus species’ genomes which grouped into gene cluster families containing BGCs linked to different carotenoid product chemotypes. Lastly, LC–MS analysis of saponified and unsaponified pigment extracts obtained from cultures of Planococcus sp. CP5-4, revealed the structure of the main (predominant) glucosylated C30- carotenoid fatty acid ester produced by Planococcus sp. CP5-4.en_US
dc.identifier.citationMoyo, A. C. et al. (2022). Structure and biosynthesis of carotenoids produced by a novel Planococcus sp. isolated from South Africa. Microbial Cell Factories, 21(1),43. https://doi.org/10.1186/s12934-022-01752-1en_US
dc.identifier.issn1475-2859
dc.identifier.urihttps://doi.org/10.1186/s12934-022-01752-1
dc.identifier.urihttp://hdl.handle.net/10566/7902
dc.language.isoenen_US
dc.publisherBMCen_US
dc.subjectPlanococcusen_US
dc.subjectHalophilic bacteriaen_US
dc.subjectCarotenoid biosynthesisen_US
dc.subjectBiotechnologyen_US
dc.subjectPigmentsen_US
dc.titleStructure and biosynthesis of carotenoids produced by a novel Planococcus sp. isolated from South Africaen_US
dc.typeArticleen_US

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