Structure and biosynthesis of carotenoids produced by a novel Planococcus sp. isolated from South Africa
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Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
BMC
Abstract
The genus Planococcus is comprised of halophilic bacteria generally reported for the production of
carotenoid pigments and biosurfactants. In previous work, we showed that the culturing of the orange-pigmented
Planococcus sp. CP5-4 isolate increased the evaporation rate of industrial wastewater brine effluent, which we attributed
to the orange pigment. This demonstrated the potential application of this bacterium for industrial brine effluent
management in evaporation ponds for inland desalination plants. Here we identified a C30-
carotenoid biosynthetic
gene cluster responsible for pigment biosynthesis in Planococcus sp. CP5-4 through isolation of mutants and genome
sequencing. We further compare the core genes of the carotenoid biosynthetic gene clusters identified from different
Planococcus species’ genomes which grouped into gene cluster families containing BGCs linked to different carotenoid
product chemotypes. Lastly, LC–MS analysis of saponified and unsaponified pigment extracts obtained from
cultures of Planococcus sp. CP5-4, revealed the structure of the main (predominant) glucosylated C30-
carotenoid fatty
acid ester produced by Planococcus sp. CP5-4.
Description
Keywords
Planococcus, Halophilic bacteria, Carotenoid biosynthesis, Biotechnology, Pigments
Citation
Moyo, A. C. et al. (2022). Structure and biosynthesis of carotenoids produced by a novel Planococcus sp. isolated from South Africa. Microbial Cell Factories, 21(1),43. https://doi.org/10.1186/s12934-022-01752-1