Inhibition of HIV-1 enzymes, antioxidant and anti-inflammatory activities of Plectranthus barbatus
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Date
213
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Ethnopharmacological relevance: Plectranthus barbatus is widely used in African countries as an herbal
remedy to manage HIV/AIDS and related conditions.
Aim of the study: To investigate the HIV-1 inhibitory, anti-inflammatory and antioxidant properties of P.
barbatus and thereby provide empirical evidence for the apparent anecdotal success of the extracts.
Materials and methods: Ethanolic extract of P. barbatus's leaves was screened against two HIV-1 enzymes:
protease (PR) and reverse transcriptase (RT). Cytotoxicity of the extract was determined through
measuring tetrazolium dye uptake of peripheral blood mononuclear cells (PBMCs) and the TZM-bl cell
line. Confirmatory assays for cytotoxicity were performed using flow cytometry and real-time cell
electronic sensing (RT-CES). The free radical scavenging activity of the extract was investigated with 2,2-
diphenyl-1-picrylhydrazyl while the anti-inflammatory properties of the plant extract were investigated
using a Th1/Th2/Th17 cytometric bead array technique.
Description
Keywords
Chemistry, HIV-1 protease, Public health, Plectranthus barbatus
Citation
Kapewangolo, P. et al. (2015). Inhibition of HIV-1 enzymes, antioxidant and anti-inflammatory activities of Plectranthus barbatus. Journal of Ethnopharmacology, 149(1),184-190. https://doi.org/10.1016/j.jep.2013.06.019