Effects of the alkaloid present in the ethyl acetate: hexane (1 :4) fraction of Crinum macowanii on the isolated perfused rat heart

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dc.contributor.advisorMugabo, Pierre
dc.contributor.authorNjagi, Angela Gakii
dc.date.accessioned2023-06-13T11:16:38Z
dc.date.accessioned2024-05-15T07:42:46Z
dc.date.available2023-06-13T11:16:38Z
dc.date.available2024-05-15T07:42:46Z
dc.date.issued2004
dc.descriptionMagister Pharmaceuticae - MPharmen_US
dc.description.abstractCrinum macowanii (CM) is used in traditional medicine in the treatment of various diseases including ischemic heart disease, rheumatic fever, cancer and skin diseases. The aqueous extract of CM bulbs was found have a positive inotropic effect similar to the one of adrenaline in normotensive rats. After the extraction of CM bulbs four fractions were collected, (1:4), (2:3), (3:2) and (4:1), from ethyl actetate: hexane as an eluent. The (1:4) further using PLC and the major band used for the experiments. Structure elucidation was further carried out on the major band isolated and a new alkaloid was identified from the bulbs of CM. The major aim of the study was to test the alkaloid isolated on the "double sided" working heart system. The parameters to be assessed were coronary flow (Qe), aortic output (Qa), cardiac output (CO), systolic and diastolic pressure (SP/DP), pulse pressure and heart rate (Hr). Wistar rats weighing between 250-350g were used. The hearts were isolated and perfused using Krebs Henseleit solution on the "double sided" working heart system. The parameters were monitored through a pressure transducer connected to a power lab and a computer. The Qe, Qa, CO, SP/DP, Pulse pressure and Hr reduced significantly when lycorinone (the proposed name given to the new alkaloid extracted from Crinium macowanii) was used at the concentrations of 0.005μg and 0.05μg. Further studies are recommended for the verification of the mechanism of action of lycorinone (negative chronotropic and negative inotropic effects).en_US
dc.identifier.urihttps://hdl.handle.net/10566/15133
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectTraditional medicineen_US
dc.subjectCardiovascularen_US
dc.subjectHearten_US
dc.subjectMedicinal herbsen_US
dc.subjectIsolated perfused hearten_US
dc.subjectWister Albino ratsen_US
dc.subjectPlant extracten_US
dc.subjectAlkaloidsen_US
dc.subjectIn vitroen_US
dc.subjectCrinum macowaniien_US
dc.titleEffects of the alkaloid present in the ethyl acetate: hexane (1 :4) fraction of Crinum macowanii on the isolated perfused rat hearten_US

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