Using multiplex amplicon pcr technology to efficiently and timely generate rift valley fever virus sequence data for genomic surveillance
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Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Rift Valley fever (RVF) is a febrile vector-borne disease endemic in Africa and continues
to spread in new territories. It is a climate-sensitive disease mostly triggered by abnormal rainfall
patterns. The disease is associated with high mortality and morbidity in both humans and livestock.
RVF is caused by the Rift Valley fever virus (RVFV) of the genus Phlebovirus in the family Phenuiviridae.
It is a tripartite RNA virus with three genomic segments: small (S), medium (M) and large (L).
Pathogen genomic sequencing is becoming a routine procedure and a powerful tool for understanding
the evolutionary dynamics of infectious organisms, including viruses. Inspired by the utility
of amplicon-based sequencing demonstrated in severe acute respiratory syndrome coronavirus-2
(SARS-CoV-2) and Ebola, Zika and West Nile viruses, we report an RVFV sample preparation based
on amplicon multiplex polymerase chain reaction (amPCR) for template enrichment and reduction
of background host contamination.
Description
Keywords
Bioinformatics, Genome, Culture, Ebola virus, Public health
Citation
Juma, J. et al. (2023). Using multiplex amplicon pcr technology to efficiently and timely generate rift valley fever virus sequence data for genomic surveillance. Viruses , 15(2), 477. https://doi.org/10.3390/v15020477