Using multiplex amplicon pcr technology to efficiently and timely generate rift valley fever virus sequence data for genomic surveillance

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dc.contributor.authorJuma, John
dc.contributor.authorKonongoi, Samson L.
dc.contributor.authorNsengimana, Isidore
dc.date.accessioned2023-04-11T09:27:56Z
dc.date.available2023-04-11T09:27:56Z
dc.date.issued2023
dc.description.abstractRift Valley fever (RVF) is a febrile vector-borne disease endemic in Africa and continues to spread in new territories. It is a climate-sensitive disease mostly triggered by abnormal rainfall patterns. The disease is associated with high mortality and morbidity in both humans and livestock. RVF is caused by the Rift Valley fever virus (RVFV) of the genus Phlebovirus in the family Phenuiviridae. It is a tripartite RNA virus with three genomic segments: small (S), medium (M) and large (L). Pathogen genomic sequencing is becoming a routine procedure and a powerful tool for understanding the evolutionary dynamics of infectious organisms, including viruses. Inspired by the utility of amplicon-based sequencing demonstrated in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and Ebola, Zika and West Nile viruses, we report an RVFV sample preparation based on amplicon multiplex polymerase chain reaction (amPCR) for template enrichment and reduction of background host contamination.en_US
dc.identifier.citationJuma, J. et al. (2023). Using multiplex amplicon pcr technology to efficiently and timely generate rift valley fever virus sequence data for genomic surveillance. Viruses , 15(2), 477. https://doi.org/10.3390/v15020477en_US
dc.identifier.issn1999-4915
dc.identifier.urihttps://doi.org/10.3390/v15020477
dc.identifier.urihttp://hdl.handle.net/10566/8728
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectBioinformaticsen_US
dc.subjectGenomeen_US
dc.subjectCultureen_US
dc.subjectEbola virusen_US
dc.subjectPublic healthen_US
dc.titleUsing multiplex amplicon pcr technology to efficiently and timely generate rift valley fever virus sequence data for genomic surveillanceen_US
dc.typeArticleen_US

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