Novel buffer for long-term preservation of DNA in biological material at room temperature

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dc.contributor.authorKasu, Mohaimin
dc.contributor.authorRistow, Peter Gustav
dc.contributor.authorBurrows, Adria Michelle
dc.date.accessioned2024-11-14T13:01:07Z
dc.date.available2024-11-14T13:01:07Z
dc.date.issued2024
dc.description.abstractThe collection and preservation of biological material before DNA analysis is critical for inter alia biomedical research, medical diagnostics, forensics and biodiversity conservation. In this study, we evaluate an in-house formulated buffer called the Forensic DNA Laboratory-buffer (FDL-buffer) for preservation of biological material for long term at room temperature. Human saliva stored in the buffer for 8 years, human blood stored for 3 years and delicate animal tissues from the jellyfish Pelagia noctiluca comb jelly Beroe sp., stored for 4 and 6 years respectively consistently produced high-quality DNA. FDL-buffer exhibited compatibility with standard organic, salting out and spin-column extraction methods, making it versatile and applicable to a wide range of applications, including automation
dc.identifier.citationKasu, M., Ristow, P.G., Burrows, A.M., Kuplik, Z., Gibbons, M.J. and D'Amato, M.E., 2024. Novel buffer for long-term preservation of DNA in biological material at room temperature. BioTechniques, 76(8), pp.357-370.
dc.identifier.urihttps://doi.org/10.33232/001c.118782
dc.identifier.urihttps://hdl.handle.net/10566/19684
dc.language.isoen
dc.publisherTaylor and Francis Ltd.
dc.subjectblood
dc.subjectDNA extraction
dc.subjectDNA preservation
dc.subjectFDL-buffer
dc.subjectjellyfish
dc.titleNovel buffer for long-term preservation of DNA in biological material at room temperature
dc.typeArticle

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