Quantum dot-sensitised estrogen receptor-α-based biosensor for 17β-estradiol

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dc.contributor.authorJijana, Abongile N.
dc.contributor.authorFeleni, Usisipho
dc.contributor.authorNdangili, Peter M.
dc.contributor.authorBilibana, Mawethu
dc.contributor.authorAjayi, Rachel F
dc.date.accessioned2023-03-16T07:38:03Z
dc.date.available2023-03-16T07:38:03Z
dc.date.issued2023
dc.description.abstract: 17β-estradiol (E2) is an important natural female hormone that is also classified as an estrogenic endocrine-disrupting compound (e-EDC). It is, however, known to cause more damaging health effects compared to other e-EDCs. Environmental water systems are commonly contaminated with E2 that originates from domestic effluents. The determination of the level of E2 is thus very crucial in both wastewater treatment and in the aspect of environmental pollution management. In this work, an inherent and strong affinity of the estrogen receptor-α (ER-α) for E2 was used as a basis for the development of a biosensor that was highly selective towards E2 determination. A gold disk electrode (AuE) was functionalised with a 3-mercaptopropionic acid-capped tin selenide (SnSe-3MPA) quantum dot to produce a SnSe-3MPA/AuE electroactive sensor platform. The ER-α-based biosensor (ER-α/SnSe-3MPA/AuE) for E2 was produced by the amide chemistry of carboxyl functional groups of SnSe-3MPA quantum dots and the primary amines of ER-α. The ER-α/SnSe-3MPA/AuE receptorbased biosensor exhibited a formal potential (E0 0 ) value of 217 ± 12 mV, assigned as the redox potential for monitoring the E2 response using square-wave voltammetry (SWV). The response parameters of the receptor-based biosensor for E2 include a dynamic linear range (DLR) value of 1.0–8.0 nM (R2 = 0.99), a limit of detection (LOD) value of 1.69 nM (S/N = 3), and a sensitivity of 0.04 µA/nM. The biosensor exhibited high selectivity for E2 and good recoveries for E2 determination in milk samplesen_US
dc.identifier.citation: Jijana, A.N.; Feleni, U.; Ndangili, P.M.; Bilibana, M.; Ajayi, R.F.; Iwuoha, E.I. Quantum Dot-Sensitised Estrogen Receptor-α-Based Biosensor for 17β-Estradiol. Biosensors 2023, 13, 242. https://doi.org/10.3390/ bios13020242en_US
dc.identifier.urihttps://doi.org/10.3390/ bios13020242
dc.identifier.urihttp://hdl.handle.net/10566/8595
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectSnSeen_US
dc.subjectHormoneen_US
dc.subjectQuantum dotsen_US
dc.subjectNanomaterialen_US
dc.subject17β-estradiol (E2)en_US
dc.subjectEndocrine-disrupting compounds (EDC)en_US
dc.titleQuantum dot-sensitised estrogen receptor-α-based biosensor for 17β-estradiolen_US
dc.typeArticleen_US

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