Quantum dot-sensitised estrogen receptor-α-based biosensor for 17β-estradiol
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Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
: 17β-estradiol (E2) is an important natural female hormone that is also classified as an
estrogenic endocrine-disrupting compound (e-EDC). It is, however, known to cause more damaging
health effects compared to other e-EDCs. Environmental water systems are commonly contaminated
with E2 that originates from domestic effluents. The determination of the level of E2 is thus very
crucial in both wastewater treatment and in the aspect of environmental pollution management. In
this work, an inherent and strong affinity of the estrogen receptor-α (ER-α) for E2 was used as a basis
for the development of a biosensor that was highly selective towards E2 determination. A gold disk
electrode (AuE) was functionalised with a 3-mercaptopropionic acid-capped tin selenide (SnSe-3MPA)
quantum dot to produce a SnSe-3MPA/AuE electroactive sensor platform. The ER-α-based biosensor
(ER-α/SnSe-3MPA/AuE) for E2 was produced by the amide chemistry of carboxyl functional groups
of SnSe-3MPA quantum dots and the primary amines of ER-α. The ER-α/SnSe-3MPA/AuE receptorbased biosensor exhibited a formal potential (E0
0
) value of 217 ± 12 mV, assigned as the redox
potential for monitoring the E2 response using square-wave voltammetry (SWV). The response
parameters of the receptor-based biosensor for E2 include a dynamic linear range (DLR) value of
1.0–8.0 nM (R2 = 0.99), a limit of detection (LOD) value of 1.69 nM (S/N = 3), and a sensitivity of
0.04 µA/nM. The biosensor exhibited high selectivity for E2 and good recoveries for E2 determination
in milk samples
Description
Keywords
SnSe, Hormone, Quantum dots, Nanomaterial, 17β-estradiol (E2), Endocrine-disrupting compounds (EDC)
Citation
: Jijana, A.N.; Feleni, U.; Ndangili, P.M.; Bilibana, M.; Ajayi, R.F.; Iwuoha, E.I. Quantum Dot-Sensitised Estrogen Receptor-α-Based Biosensor for 17β-Estradiol. Biosensors 2023, 13, 242. https://doi.org/10.3390/ bios13020242