The development of l-phenylalanine biosensor using boron-doped diamond (bdd) electrode modified with poly aniline-polyvinyl sulphonate composite film
| dc.contributor.advisor | Baker, Priscilla G.L. | |
| dc.contributor.author | Mangombo, Zelo | |
| dc.date.accessioned | 2023-06-12T13:01:52Z | |
| dc.date.accessioned | 2024-05-09T10:51:20Z | |
| dc.date.available | 2023-06-12T13:01:52Z | |
| dc.date.available | 2024-05-09T10:51:20Z | |
| dc.date.issued | 2010 | |
| dc.description | >Magister Scientiae - MSc | en_US |
| dc.description.abstract | The aim of this project was to develop a biosensor on a modified boron-doped diamond (BDD) platform in order to determine L-phenylalanine (L-Phe) levels in the aqueous media and real samples. A BDD electrode modified with polyaniline (PANI) film doped with polyvinyl sulphonate (PVS), containing in-situ deposited tyrosinase (Tyr) enzyme was studied with special emphasis on the detection of L-phenylalanine (L-Phe). The PANI-PVS film was electrochemically coated on the BDD surface by cyclic voltammetry (CV) at 50 mV s-1 (versus Ag/AgCI). An increase in current density with increasing number of cycles was an indication of the polymer growth. The morphologies of the composite films were characterized by scanning electron microscopy (SEM). | en_US |
| dc.identifier.uri | https://hdl.handle.net/10566/14596 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of the Western Cape | en_US |
| dc.rights.holder | University of the Western Cape | en_US |
| dc.subject | Polyaniline | en_US |
| dc.subject | Electrochemical enzyme biosensor | en_US |
| dc.subject | Chemistry | en_US |
| dc.subject | Cyclic voltammetry | en_US |
| dc.subject | Lipropotein | en_US |
| dc.title | The development of l-phenylalanine biosensor using boron-doped diamond (bdd) electrode modified with poly aniline-polyvinyl sulphonate composite film | en_US |