The development of l-phenylalanine biosensor using boron-doped diamond (bdd) electrode modified with poly aniline-polyvinyl sulphonate composite film
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Date
2010
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Publisher
University of the Western Cape
Abstract
The aim of this project was to develop a biosensor on a modified boron-doped diamond (BDD)
platform in order to determine L-phenylalanine (L-Phe) levels in the aqueous media and real
samples. A BDD electrode modified with polyaniline (PANI) film doped with polyvinyl
sulphonate (PVS), containing in-situ deposited tyrosinase (Tyr) enzyme was studied with special
emphasis on the detection of L-phenylalanine (L-Phe). The PANI-PVS film was
electrochemically coated on the BDD surface by cyclic voltammetry (CV) at 50 mV s-1 (versus
Ag/AgCI). An increase in current density with increasing number of cycles was an indication of
the polymer growth. The morphologies of the composite films were characterized by scanning
electron microscopy (SEM).
Description
>Magister Scientiae - MSc
Keywords
Polyaniline, Electrochemical enzyme biosensor, Chemistry, Cyclic voltammetry, Lipropotein