Measuring Streptococcus mutans, Streptococcus sanguinis and Candida albicans biofilm formation using a real-time impedance-based system

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dc.contributor.authorAbrantes, Pedro Miguel dos Santos
dc.contributor.authorAfrica, Charlene Wilma Joyce
dc.date.accessioned2020-10-30T08:59:36Z
dc.date.available2020-10-30T08:59:36Z
dc.date.issued2019-12-20
dc.description.abstractCandida albicans and streptococci are amongst the most common fungal and bacterial organisms present in the oral cavity, with a growing body of evidence implicating C. albicans in increased caries severity and in the formation of the cariogenic biofilm. However, the interactive mechanisms between cariogenic streptococci and Candida are yet to be elucidated. In this study, the real-time biofilm formation of C. albicans, S. mutans and S. sanguinis was assessed individually and in combination using the xCELLigence system, an impedance-based microbial biofilm monitoring system. The impedance signal was the highest for C. albicans, followed by S. mutans and S. sanguinis. Although the streptococcal mixed adhesion was found to follow a similar trend to that of S. sanguinis, the introduction of C. albicans resulted in higher adhesion patterns, with the combined growth of S. sanguinis and C. albicans and the combination of all three species resulting in higher biofilm formation than any of the individual organisms over time. This study, the first to use impedance for real-time monitoring of interkingdom biofilms, adds to the body of evidence that C. albicans and oral streptococcal adhesion are interlinked and suggests that interkingdom interactions induce changes in the oral biofilm dynamics over time.en_US
dc.description.sponsorshipNational Research Foundation of South Africaen_US
dc.identifier.citationPedro MDS Abrantes, Charlene WJ Africa. 2020. Measuring Streptococcus mutans, Streptococcus sanguinis and Candida albicans biofilm formation using a real-time impedance-based system. J Microbiol Methods 169:105815en_US
dc.identifier.issn0167-7012
dc.identifier.otherdoi: 10.1016/j.mimet.2019.105815
dc.identifier.urihttp://hdl.handle.net/10566/5337
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectCandida albicansen_US
dc.subjectStreptococcus mutansen_US
dc.subjectStreptococcus sanguinisen_US
dc.subjectOral biofilmsen_US
dc.subjectCell adhesionen_US
dc.subjectxCELLigence systemen_US
dc.titleMeasuring Streptococcus mutans, Streptococcus sanguinis and Candida albicans biofilm formation using a real-time impedance-based systemen_US
dc.typeArticleen_US

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