Measuring Streptococcus mutans, Streptococcus sanguinis and Candida albicans biofilm formation using a real-time impedance-based system
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Date
2019-12-20
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Candida albicans and streptococci are amongst the most common fungal and bacterial organisms present in the
oral cavity, with a growing body of evidence implicating C. albicans in increased caries severity and in the
formation of the cariogenic biofilm. However, the interactive mechanisms between cariogenic streptococci and
Candida are yet to be elucidated. In this study, the real-time biofilm formation of C. albicans, S. mutans and S.
sanguinis was assessed individually and in combination using the xCELLigence system, an impedance-based
microbial biofilm monitoring system. The impedance signal was the highest for C. albicans, followed by S. mutans
and S. sanguinis. Although the streptococcal mixed adhesion was found to follow a similar trend to that of S.
sanguinis, the introduction of C. albicans resulted in higher adhesion patterns, with the combined growth of S.
sanguinis and C. albicans and the combination of all three species resulting in higher biofilm formation than any
of the individual organisms over time. This study, the first to use impedance for real-time monitoring of interkingdom
biofilms, adds to the body of evidence that C. albicans and oral streptococcal adhesion are interlinked
and suggests that interkingdom interactions induce changes in the oral biofilm dynamics over time.
Description
Keywords
Candida albicans, Streptococcus mutans, Streptococcus sanguinis, Oral biofilms, Cell adhesion, xCELLigence system
Citation
Pedro MDS Abrantes, Charlene WJ Africa. 2020. Measuring Streptococcus mutans, Streptococcus sanguinis and Candida albicans biofilm formation using a real-time impedance-based system. J Microbiol Methods 169:105815