Browsing by Author "Kasu, Mohaimin"

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    Novel buffer for long-term preservation of DNA in biological material at room temperature
    (Taylor and Francis Ltd., 2024) Kasu, Mohaimin; Ristow, Peter Gustav; Burrows, Adria Michelle
    The collection and preservation of biological material before DNA analysis is critical for inter alia biomedical research, medical diagnostics, forensics and biodiversity conservation. In this study, we evaluate an in-house formulated buffer called the Forensic DNA Laboratory-buffer (FDL-buffer) for preservation of biological material for long term at room temperature. Human saliva stored in the buffer for 8 years, human blood stored for 3 years and delicate animal tissues from the jellyfish Pelagia noctiluca comb jelly Beroe sp., stored for 4 and 6 years respectively consistently produced high-quality DNA. FDL-buffer exhibited compatibility with standard organic, salting out and spin-column extraction methods, making it versatile and applicable to a wide range of applications, including automation
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    UniQ-Typer ™ Y-10 genotyping in South African populations: novel alleles, sequence variation and allelic ladder updates
    (Elsevier, 2019) Kasu, Mohaimin; Fraser, Mischa; D’Amato, Maria Eugenia
    DNA profiling the male specific region on the Y-chromosome is fundamental to forensic practise. It is recognised as a powerful tool for sexual assault investigations for resolving the male DNA components, for familial search and as a tool to aid biogeographic inferences. In this study the novel Y-chromosome Short Tandem Repeat (Y-STR) multiplex called the UniQ-Typer ™ Y-10 was utilised for population wide genotyping in South Africa. In this study we provide a significant updated to the genotyping allelic ladder for the panel and report novel variation which gave strong signals to potentially infer biogeographic ancestry
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    Validation and application of a highly discriminating and rapid 10-locus Y-STR DNA profiling system
    (University of the Western Cape, 2019) Kasu, Mohaimin; D’Amato, Maria Eugenia
    DNA profiling the male specific region on the Y-chromosome is fundamental to forensic practise. Its recognised as a powerful analytical tool for investigation of sexual assault when the DNA evidence is highly admixed. Standard practises for processing sexual assault evidence include physically separate the sperm cell from the female fraction using differential extraction followed by autosomal DNA profiling. However, under specific scenarios of assault physical separation may not be possible due to the nature of the evidence. The research presented in this thesis was focused on the development and validation of the UniQTyper™ Y-10 prototype for male specific DNA profiling. The prototype which contains 10 Y-STR markers was developed and validated to deliver a rapid and cost-effective system while maintaining a forensic applicable level of performance. An allelic ladder is produced with an allele cloning approach for which an overview of the workflow and technicalities presented herein is aimed to assists an efficient bulk production process. In a second component novel sequence variation was reported across 153 sequenced alleles and submitted to Genbank. In this output the Y-STR panel was perused beyond the scope of length polymorphisms. In a proof of concept, its potential to discriminate between shared allele sizes by characterizing sequence structure variations is discussed. In a final component we generate the largest Y-STR survey across South Africa to establish reference data and to comprehensively assess the forensic genetics parameters for the UniQTyper™ Y-10.